A novel fucolectin from Apostichopus japonicus with broad PAMP recognition pattern
A novel fucolectin from Apostichopus japonicus with broad PAMP recognition pattern
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具有广泛 PAMP 识别模式的仿刺参新型岩藻凝集素
DOI：
10.1016/j.fsi.2018.04.013
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发表时间：
2018-06
影响因子：
4.7
通讯作者：
Song Linsheng
中科院分区：
文献类型：
--
作者：
Wang Ying;Xue Zhuang;Yi Qilin;Wang Hui;Wang Lingling;Lu Guangxia;Liu Yu;Qu Chen;Li Yannan;Song Linsheng
F-型凝集素（也称为岩藻凝集素）是一个新发现的具有岩藻糖结合基序和独特的凝集素折叠（"F-型"折叠）的岩藻糖结合凝集素家族。本研究从仿刺参（Apostichopus）中分离鉴定了一种岩藻凝集素（AjFL-1）。AjFL-1基因的开放阅读框（ORF）为546 bp，编码181个氨基酸，预测分子量约为20 kDa。AjFL-1的氨基酸序列与其它动物的岩藻凝集素有30%~40%的相似性。AjFL-1具有典型的F型凝集素结构域（FLD）（第39 - 180位残基）和信号肽（第1 - 24位残基）。qRT-PCR检测到AjFL-1mRNA在生殖腺、体腔细胞、呼吸树、腱、体壁等组织中均有表达，而在性腺和纵肌中未检测到。在灿烂弧菌攻击后12 h，体腔细胞AjFL-1mRNA表达水平显著上调（是对照组的47.06倍，p <0.05）。免疫荧光检测结果表明，AjFL-1蛋白主要分布在海参体腔细胞的膜上，而在细胞质中的分布较少。重组AjFL-1（rAjFL-1）可与脂多糖（LPS）、肽聚糖（PGN）、甘露聚糖（MAN）和岩藻糖（FUC）结合，并对革兰氏阴性细菌大肠杆菌、革兰氏阳性细菌藤黄微球菌以及真菌巴斯德毕赤酵母表现出更广泛的结合活性。此外，rAjFL-1对真菌P.帕斯托里斯。这些结果表明，AjFL-1是一个新的岩藻糖结合凝集素家族成员，是一种具有广谱微生物识别功能的模式识别受体，参与了海参的天然免疫应答。
F-type lectin (also known as fucolectin) is a newly identified family of fucose binding lectins with the sequence characters of a fucose binding motif and a unique lectin fold (the “F-type” fold). In the present study, a fucolectin was identified from sea cucumber Apostichopus japonicus (designatedAjFL-1). The open reading frame (ORF) ofAjFL-1 was of 546 bp, encoding a polypeptide of 181 amino acids with a predicted molecular mass of about 20 kDa. The deduced amino acid sequence ofAjFL-1 shared 30%-40% similarity with the fucolectins from other animals. There were a typical F-type lectin domain (FLD) (residues 39-180) and a signal peptide (residues 1-24) inAjFL-1. The mRNA transcript ofAjFL-1 could be detected by qRT-PCR in various tissues, such as intestinum, coelomocytes, respiratory tree, tentacle, and body wall, while undetectable in the gonads and longitudinal muscle. The mRNA expression level ofAjFL-1 in coelomocytes was significantly up-regulated (47.06-fold to that in control group,p< 0.05) at 12 h after Vibrio splendidus challenge. Immunofluorescence assay showed thatAjFL-1 protein was mainly distributed on the membrane, while few in cytoplasm of coelomocytes in sea cucumber. The recombinantAjFL-1 (rAjFL-1) could bind lipopolysaccharide (LPS), peptidoglycan (PGN), mannan (MAN) and fucose (FUC), and exhibited a broader binding activities towards Gram-negative bacteriumEscherichia coli, Gram-positive bacterium Micrococcus luteus, as well fungus Pichia pastoris. In addition, rAjFL-1 could strongly promote the agglutination of fungusP. pastoris. These results indicated thatAjFL-1 was a novel member of fucose-binding lectin family, which functioned as a pattern recognition receptor with broad spectrum of microbial recognition, and involved in innate immune response of sea cucumber.
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影响因子：
2.9
作者：
Vasta, Gerardo R.;Nita-Lazar, Mihai;Giomarelli, Barbara;Ahmed, Hafiz;Du, Shaojun;Cammarata, Matteo;Parrinello, Nicolo;Bianchet, Mario A.;Amzel, L. Mario
通讯作者：
Amzel, L. Mario
影响因子：
4.7
作者：
Salerno, G.;Parisi, M. G.;Cammarata, M.
通讯作者：
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影响因子：
0.7
作者：
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通讯作者：
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影响因子：
2.9
作者：
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通讯作者：
H. R. Williams;B. Macey;L. Burnett;K. Burnett
斑马鱼气溶素样蛋白受体识别和孔形成的结构基础
影响因子：
7.7
作者：
Jia, Ning;Liu, Nan;Zhou, Cong-Zhao
通讯作者：
Zhou, Cong-Zhao