Image Contrast in Confocal Light Microscopy

Image Contrast in Confocal Light Microscopy
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共焦光学显微镜中的图像对比度

DOI:
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发表时间:
1990
期刊:
影响因子:
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通讯作者:
A. Kriete
A. Kriete
中科院分区:
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文献类型:
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作者:
P. Cheng;A. Kriete

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对于任何形式的显微镜,人们不仅需要一个具有足够分辨率的成像系统来揭示样本的精细细节,而且还需要一个合适的对比机制来“看到”感兴趣的结构。根据《新韦氏词典》的定义,对比度是底片或印刷品的明暗区域之间的差异。换句话说,对比度是图像不同部分之间的信号强度差异,或者是感兴趣的细节和“背景”之间的信号强度差异。更科学地说,检测到的对比度与两个图像区域之间的亮度差Ai除以平均图像亮度Ī成正比。
For any form of microscopy, one needs not only an imaging system which has enough resolution to reveal the fine details of a specimen, but also, a suitable contrast mechanism to “see” the structures of interest. As defined by the New Webster’s Dictionary, contrast is the difference between light and dark areas of a negative or print. In other words, contrast is the difference in signal strength between various parts of an image or between details of interest and “background”. To be slightly more scientific, the detected contrast is proportional to the brightness difference, A I, between two image areas divided by the average image brightness, Ī.
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DOI: 10.1167/iovs.61.6.25
发表时间: 2020
影响因子: 4.4
作者:
Ledolter,Johannes;Gramlich,OliverW;Kardon,RandyH
通讯作者: Kardon,RandyH