De novo synthesis of Legionella pneumophila antigens during intracellular growth in phagocytic cells
De novo synthesis of Legionella pneumophila antigens during intracellular growth in phagocytic cells
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吞噬细胞细胞内生长过程中嗜肺军团菌抗原的从头合成
DOI：
10.1128/iai.64.5.1679-1684.1996
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发表时间：
1996
影响因子：
3.1
通讯作者：
R. Marre
中科院分区：
文献类型：
--
作者：
M. Šuša;J. Hacker;R. Marre
嗜肺军团菌是一种革兰氏阴性杆菌，能够在吞噬细胞内繁殖。吞噬过程导致环境的快速变化，这可能需要对基因表达进行协调调节，以确保细胞内的生存。由于吞噬早期基因上调和下调的信息很少，我们在Mono Mac 6细胞系的巨噬细胞吞噬后的不同时间对嗜肺乳杆菌进行了放射性标记，并用抗军团菌血清或单抗进行了免疫沉淀。我们可以鉴定出两个上调的抗原，其中一个是MIP蛋白，三个下调的抗原，以及三个在细胞外培养的嗜肺乳杆菌中不能检测到的抗原。MIP蛋白在细胞内感染后4~8h染色最强，提示MIP蛋白在细胞内增殖过程中是必需的，而不是在感染开始时。以Mono Mac 6细胞为吞噬细胞，感染后2~4h，胞外生长过程中未检测到的44 kDa抗原最为明显。44 kDa抗原在卡氏棘阿米巴、MRC-5和U937细胞的生长过程中也有表达，但表达动力学不同。该抗原的合成不依赖于宿主细胞的蛋白质合成。由于44 kDa的抗血清可以被含有嗜肺乳杆菌插入片段的重组大肠杆菌的抗血清沉淀，因此我们认为相应的基因位于MIP基因的附近。我们将这种蛋白命名为军团菌胞内生长抗原(LIGA)，因为它只存在于细胞内培养的嗜肺乳杆菌中。
Legionella pneumophilia is a gram-negative rod which is able to multiply within phagocytic cells. The process of phagocytosis leads to a rapid environmental change that might require a coordinate regulation of gene expression to ensure intracellular survival. Since there is little information on up- and downregulation of genes during the early phases of phagocytosis, we radiolabeled intracellular L. pneumophila at different times after phagocytosis by macrophages of the Mono Mac 6 cell line and immunoprecipitated antigens with antilegionella sera or monoclonal antibodies. We could identify two antigens which were upregulated, one of which was the Mip protein, three antigens which were downregulated, and three antigens which were not detectable in extracellularly grown L. pneumophila. The Mip protein was stained most intensively 4 to 8 h after intracellular infection, suggesting that it is needed during intracellular multiplication rather than initiation of infection. A 44-kDa antigen which was not detectable during extracellular growth was most prominent from 2 to 4 h postinfection when Mono Mac 6 cells were used as phagocytic cells. The 44-kDa antigen was also expressed during growth with Acanthamoeba castelanii, MRC-5, and U937 cells but with different kinetics. Synthesis of this antigen was not dependent on protein synthesis of the host cell. Since the 44-kDa antigen could be precipitated by an antiserum produced against a recombinant Escherichia coli harboring a plasmid with an L. pneumophila insert which also codes for the mip gene, we believe that the corresponding gene is within the vicinity of the mip gene. We named this protein legionella intracellular growth antigen (LIGA), since it could be found exclusively in intracellularly grown L. pneumophila.
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影响因子：
15.9
作者：
BYRD, TF;HORWITZ, MA
通讯作者：
HORWITZ, MA
编码嗜肺军团菌 25 kDa 主要外膜蛋白 (MOMP) 的基因 (ompM) 的克隆、核苷酸序列和在大肠杆菌中的表达。
DOI：
10.1099/00221287-139-8-1715
发表时间：
1993
期刊：
Journal of general microbiology
影响因子：
--
作者：
High,AS;Torosian,SD;Rodgers,FG
通讯作者：
Rodgers,FG
DOI：
10.1073/pnas.89.11.5188
发表时间：
1992-06-01
影响因子：
11.1
作者：
CIANCIOTTO, NP;FIELDS, BS
通讯作者：
FIELDS, BS
DOI：
10.1073/pnas.89.20.9607
发表时间：
1992-10-15
影响因子：
11.1
作者：
MARRA, A;BLANDER, SJ;SHUMAN, HA
通讯作者：
SHUMAN, HA
影响因子：
6.4
作者：
CIANCIOTTO, NP;EISENSTEIN, BI;ENGLEBERG, NC
通讯作者：
ENGLEBERG, NC