The genetic makeup of the Drosophila piRNA pathway.
The genetic makeup of the Drosophila piRNA pathway.
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DOI：
10.1016/j.molcel.2013.04.031
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发表时间：
2013-06-06
期刊：
影响因子：
16
通讯作者：
Brennecke, Julius
中科院分区：
文献类型：
--
作者：
Handler, Dominik;Meixner, Katharina;Pizka, Manfred;Lauss, Kathrin;Schmied, Christopher;Gruber, Franz Sebastian;Brennecke, Julius
PiRNA(PIWI-Interaction RNA)途径是一个小的RNA沉默系统，作用于动物的性腺，保护基因组免受转座子的有害影响。该领域的一个主要瓶颈是缺乏对构成这一途径的因素和分子过程的全面了解。我们在果蝇中进行了RNA干扰筛选，并确定了强烈影响卵巢体细胞∼途径的RNA50基因。许多已识别的基因属于功能类别，表明在该途径中对线粒体新陈代谢、RNA输出、核孔、转录延伸和染色质调节具有重要作用。对两个因子的后续研究表明，在该途径的不同层级水平上作用的成分被识别出来。最后，我们将CG2183/GASZ定义为体细胞和生殖细胞中必需的主要piRNA生物发生因子。基于昆虫和脊椎动物piRNA途径的相似性，我们的结果对于理解这种保守的基因组防御系统具有深远的意义。果蝇体细胞piRNA途径因子的系统鉴定piRNA生物学与主要细胞过程之间功能联系的鉴定CG2183/GASZ是一种重要的piRNA生物发生因子
The piRNA (PIWI-interacting RNA) pathway is a small RNA silencing system that acts in animal gonads and protects the genome against the deleterious influence of transposons. A major bottleneck in the field is the lack of comprehensive knowledge of the factors and molecular processes that constitute this pathway. We conducted an RNAi screen in Drosophila and identified ∼50 genes that strongly impact the ovarian somatic piRNA pathway. Many identified genes fall into functional categories that indicate essential roles for mitochondrial metabolism, RNA export, the nuclear pore, transcription elongation, and chromatin regulation in the pathway. Follow-up studies on two factors demonstrate that components acting at distinct hierarchical levels of the pathway were identified. Finally, we define CG2183/Gasz as an essential primary piRNA biogenesis factor in somatic and germline cells. Based on the similarities between insect and vertebrate piRNA pathways, our results have far-reaching implications for the understanding of this conserved genome defense system. Systematic identification of somatic piRNA pathway factors in Drosophila Identification of functional links between piRNA biology and major cellular processes Characterization of CG2183/Gasz as an essential primary piRNA biogenesis factor
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影响因子：
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作者：
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影响因子：
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果蝇HP1同源性犀牛是通过双链簇产生的转座子沉默和PIRNA所必需的。
影响因子：
64.5
作者：
Klattenhoff C;Xi H;Li C;Lee S;Xu J;Khurana JS;Zhang F;Schultz N;Koppetsch BS;Nowosielska A;Seitz H;Zamore PD;Weng Z;Theurkauf WE
通讯作者：
Theurkauf WE