A 1-Cys Peroxiredoxin from a Thermophilic Archaeon Moonlights as a Molecular Chaperone to Protect Protein and DNA against Stress-Induced Damage.
A 1-Cys Peroxiredoxin from a Thermophilic Archaeon Moonlights as a Molecular Chaperone to Protect Protein and DNA against Stress-Induced Damage.
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来自嗜热古细菌的 1-Cys 过氧化还原蛋白同时作为分子伴侣保护蛋白质和 DNA 免受压力引起的损伤
DOI：
10.1371/journal.pone.0125325
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Cheong GW
中科院分区：
文献类型：
--
作者：
Lee S;Jia B;Liu J;Pham BP;Kwak JM;Xuan YH;Cheong GW
过氧化物酶（Prxs）作用于过氧化氢（H2 O2）、有机过氧化物和过氧亚硝酸盐。Thermococcus kodakaraensis KOD 1是一种厌氧古菌，含有多种抗氧化蛋白，包括三种Prxs（Tk 0537、Tk 0815和Tk 1055）。只有Tk 0537被发现在热、渗透压和氧化应激反应中被诱导。Tk 0537属于1-Cys Prx 6亚家族，命名为1-Cys TkPrx。使用凝胶过滤色谱法，电子显微镜，和蓝色的天然聚丙烯酰胺凝胶电泳，我们观察到，1-半胱氨酸TkPrx表现出低聚物的形式，减少过氧化物还原酶活性，以及十聚体和十二聚体的形式，可以作为分子伴侣，保护蛋白质和DNA的氧化应激。突变分析表明，在N-末端的半胱氨酸残基（Cys 46）负责过氧化物还原酶活性，和在C-末端的半胱氨酸残基（Cys 205和Cys 211）是重要的寡聚化。基于我们的研究结果，我们建议，不同的寡聚体之间的相互转换是重要的1-Cys TkPrx的不同功能的调节。
Peroxiredoxins (Prxs) act against hydrogen peroxide (H2O2), organic peroxides, and peroxynitrite. Thermococcus kodakaraensis KOD1, an anaerobic archaeon, contains many antioxidant proteins, including three Prxs (Tk0537, Tk0815, and Tk1055). Only Tk0537 has been found to be induced in response to heat, osmotic, and oxidative stress. Tk0537 was found to belong to a 1-Cys Prx6 subfamily based on sequence analysis and was named 1-Cys TkPrx. Using gel filtration chromatography, electron microscopy, and blue-native polyacrylamide gel electrophoresis, we observed that 1-Cys TkPrx exhibits oligomeric forms with reduced peroxide reductase activity as well as decameric and dodecameric forms that can act as molecular chaperones by protecting both proteins and DNA from oxidative stress. Mutational analysis showed that a cysteine residue at the N-terminus (Cys46) was responsible for the peroxide reductase activity, and cysteine residues at the C-terminus (Cys205 and Cys211) were important for oligomerization. Based on our results, we propose that interconversion between different oligomers is important for regulating the different functions of 1-Cys TkPrx.
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影响因子：
6.9
作者：
König J;Galliardt H;Jütte P;Schäper S;Dittmann L;Dietz KJ
通讯作者：
Dietz KJ
DOI：
10.1073/pnas.91.15.7017
发表时间：
1994-07-19
影响因子：
11.1
作者：
CHAE, HZ;ROBISON, K;RHEE, SG
通讯作者：
RHEE, SG
DOI：
10.1006/bbrc.1993.1410
发表时间：
1993-04-15
影响因子：
3.1
作者：
LIM, YS;CHA, MK;KIM, IH
通讯作者：
KIM, IH
影响因子：
5.6
作者：
Kim, KI;Cheong, GW;Chung, CH
通讯作者：
Chung, CH
影响因子：
5.7
作者：
Saccoccia, Fulvio;Di Micco, Patrizio;Boumis, Giovanna;Brunori, Maurizio;Koutris, Ilias;Miele, Adriana E.;Morea, Veronica;Sriratana, Palita;Williams, David L.;Bellelli, Andrea;Angelucci, Francesco
通讯作者：
Angelucci, Francesco