Cell death of motoneurons in the chick embryo spinal cord. VI. Reduction of naturally occurring cell death in the thoracolumbar column of terni by nerve growth factor-猫眼课题宝

鸡胚脊髓中运动神经元的细胞死亡。

DOI：

发表时间：

1982

期刊：

The Journal of comparative neurology

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

R. Oppenheim;J. Maderdrut;D. Wells

在第8天的禽类胚胎中，在尾侧胸腰段（节段21-23）脊髓中的离散的节前细胞柱（Terni柱，CT）首次可辨别，此时其含有约9，300个内脏运动神经元。到第10天，在胸腰椎（交感神经）CT中有大约6，900个运动神经元，并且该数量保持恒定直到至少第15天。从第3天至第9天，在绒毛尿囊膜上每日注射神经生长因子（NGF）（1-20 μg），导致第10天相应CT中尾侧胸腰交感神经节体积和运动神经元数量呈剂量依赖性增加。脊旁交感神经节神经元的数量和体积也增加。神经生长因子减少了第8天和第10天胸腰交感神经节和相应CT中固缩（死亡）神经元的数量。在长期给予NGF后，胸腰交感神经节和相应CT中的神经元数量增加是细胞死亡减少的结果。最高剂量的神经生长因子减少自然发生的细胞死亡在胸腰椎CT超过60%。从第3天到第9天每天注射细胞色素C（20 μg）对第10天CT中交感神经节的体积或运动神经元的数量没有影响

A discrete preganglionic cell column (column of Terni, CT) in the caudal thoracolumbar (segments 21–23) spinal cord is first discernible in avian embryos on day 8 when it contains approximately 9,300 visceral motoneurons. By day 10 there are about 6,900 motoneurons in the thoracolumbar (sympathetic) CT and this number remains constant until at least day 15. Daily injections of nerve growth factor (NGF) (1–20 μg) on the chorioallantoic membrane from day 3 to day 9 produced a dose‐dependent increase in both the volume of the caudal thoracolumbar sympathetic ganglia and the number of motoneurons in the corresponding CT on day 10. The number and size of the neurons in the paravertebral sympathetic ganglia was also increased by NGF. Nerve growth factor decreased the number of pyknotic (dying) neurons in both the thoracolumbar sympathetic ganglia and the corresponding CT on days 8 and 10. The increased number of neurons in both the thoracolumbar sympathetic ganglia and the corresponding CT following chronic administration of NGF is the result of decreased cell death. The highest dose of NGF reduced naturally occurring cell death in the thoracolumbar CT by more than 60%. Daily injections of cytochrome C (20 μg) from day 3 to day 9 had no effect on either the volume of the sympathetic ganglia or the number of motoneurons in the CT on day 10

Catecholamine biosynthetic enzymes are expressed in replicating cells of the peripheral but not the central nervous system.