High quality de novo sequencing and assembly of the Saccharomyces arboricolus genome.
High quality de novo sequencing and assembly of the Saccharomyces arboricolus genome.
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DOI：
10.1186/1471-2164-14-69
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发表时间：
2013-01-31
期刊：
影响因子：
4.4
通讯作者：
Nieduszynski CA
中科院分区：
文献类型：
--
作者：
Liti G;Nguyen Ba AN;Blythe M;Müller CA;Bergström A;Cubillos FA;Dafhnis-Calas F;Khoshraftar S;Malla S;Mehta N;Siow CC;Warringer J;Moses AM;Louis EJ;Nieduszynski CA
比较基因组学是一个强大的工具，以确定整个基因组的功能元件。在过去的十年里，在芽殖酵母酿酒酵母和一组密切相关的物种的研究已经在显示序列保守性分析模式的好处。增加密切相关的基因组序列的数量使得比较基因组学方法更加强大和准确。本文报道了一种与S. arboricolus有密切关系的酵母菌株Saccharomyces arboricolus的基因组序列和分析结果。啤酒。我们获得了高质量的从头序列和组装使用下一代测序技术的组合，建立了该物种的系统发育位置，并考虑了其表型在多种环境条件下的基因含量和遗传。我们认为S. Arboricolus的基因组学研究将有助于严格意义上的酵母菌的比较基因组学分析。
Comparative genomics is a formidable tool to identify functional elements throughout a genome. In the past ten years, studies in the budding yeast Saccharomyces cerevisiae and a set of closely related species have been instrumental in showing the benefit of analyzing patterns of sequence conservation. Increasing the number of closely related genome sequences makes the comparative genomics approach more powerful and accurate. Here, we report the genome sequence and analysis of Saccharomyces arboricolus, a yeast species recently isolated in China, that is closely related to S. cerevisiae. We obtained high quality de novo sequence and assemblies using a combination of next generation sequencing technologies, established the phylogenetic position of this species and considered its phenotypic profile under multiple environmental conditions in the light of its gene content and phylogeny. We suggest that the genome of S. arboricolus will be useful in future comparative genomics analysis of the Saccharomyces sensu stricto yeasts.
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