Complete Mitochondrial and Plastid DNA Sequences of the Freshwater Green Microalga <i>Medakamo hakoo</i>

Complete Mitochondrial and Plastid DNA Sequences of the Freshwater Green Microalga <i>Medakamo hakoo</i>
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淡水绿色微藻 <i>Medakamo hakoo</i> 的完整线粒体和质体 DNA 序列

DOI:
10.1101/2021.07.27.453968
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发表时间:
2021
期刊:
bioRxiv
影响因子:
--
通讯作者:
Misumi Osami
Misumi Osami
中科院分区:
--
文献类型:
--
作者:
Takusagawa Mari;Kato Shoichi;Matsunaga Sachihiro;Maruyama Shinichiro;Tsujimoto-Inui Yayoi;Nozaki Hisayoshi;Yagisawa Fumi;Ohnuma Mio;Kuroiwa Haruko;Kuroiwa Tsuneyoshi;Misumi Osami

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我们报告了一个超小的绿色藻类,Medakamo hakoo株M-hakoo 311,它具有最小的已知核基因组在淡水绿色藻类的完整细胞器基因组序列。Medakamo hakoo具有90.8-kb的叶绿体和36.5-kb的线粒体基因组,分别含有80和33个推定的蛋白质编码基因。线粒体基因组是迄今为止研究的Trebouxiophyceae藻类中最小的。核基因组的GC含量为73%,而叶绿体和线粒体基因组的GC含量分别为41%和35%。细胞器基因组密码子的使用与核基因组密码子的使用有不同的趋势。细胞器基因组具有独特的特征,如线粒体基因在单链上的偏向编码和叶绿体核糖体基因中没有操纵子结构。这将有助于理解细胞器基因组的进化以及叶绿体和线粒体中基因表达的调控。
We report the complete organellar genome sequences of an ultrasmall green alga, Medakamo hakoo strain M-hakoo 311, which has the smallest known nuclear genome in freshwater green algae. Medakamo hakoo has 90.8-kb chloroplast and 36.5-kb mitochondrial genomes containing 80 and 33 putative protein-coding genes, respectively. The mitochondrial genome is the smallest in the Trebouxiophyceae algae studied so far. The GC content of the nuclear genome is 73%, but those of chloroplast and mitochondrial genomes are 41% and 35%, respectively. Codon usages in the organellar genomes have a different tendency from that in the nuclear genome. The organellar genomes have unique characteristics, such as the biased encoding of mitochondrial genes on a single strand and the absence of operon structures in chloroplast ribosomal genes. Medakamo hakoo will be helpful for understanding the evolution of the organellar genome and the regulation of gene expression in chloroplasts and mitochondria.
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