Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profiling.
Novel insights into the relationships between dendritic cell subsets in human and mouse revealed by genome-wide expression profiling.
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DOI：
10.1186/gb-2008-9-1-r17
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发表时间：
2008-01-24
期刊：
影响因子：
12.3
通讯作者：
Dalod, Marc
中科院分区：
文献类型：
--
作者：
Robbins, Scott H.;Walzer, Thierry;Dembele, Doulaye;Thibault, Christelle;Defays, Axel;Bessou, Gilles;Xu, Huichun;Vivier, Eric;Sellars, MacLean;Pierre, Philippe;Sharp, Franck R.;Chan, Susan;Kastner, Philippe;Dalod, Marc
小鼠和人类白细胞的全基因组表达谱揭示了类浆细胞或传统树突细胞亚群的保守转录程序。树突状细胞（DC）是一组复杂的细胞，在脊椎动物免疫中发挥着关键作用。淋巴结驻留 DC (LN-DC) 又分为传统 DC (cDC) 亚群（小鼠中的 CD11b 和 CD8α；人类中的 BDCA1 和 BDCA3）和浆细胞样 DC (pDC)。目前尚不清楚这些不同的 DC 群体是否属于独特的造血谱系，以及在小鼠和人类系统中鉴定的亚群是否是进化同源物。为了获得对这些问题的新见解，我们通过分析小鼠或人类白细胞的全基因组表达谱概要，寻找 LN-DC 和体外衍生的粒细胞-巨噬细胞集落刺激因子 (GM-CSF) DC 的保守遗传特征。我们通过聚类分析表明，所有 LN-DC 亚群在白细胞家谱中形成一个独特的分支，并揭示了所有 LN-DC 亚群中进化保守的转录特征。此外，我们还确定了小鼠和人类 pDC 之间共享的大型基因表达程序，以及小鼠和人类 LN-cDC 亚群之间共享的较小保守型谱。重要的是，这些基因中的大多数先前并未与 DC 功能相关，并且许多基因具有未知的功能。最后，我们利用概要分析重新评估了产生干扰素的杀伤DC、lin-CD16+HLA-DR+细胞和体外衍生的GM-CSF DC的分类，并表明这些细胞分别与自然杀伤细胞和骨髓细胞联系更密切。我们的研究为未来研究控制白细胞亚群（尤其是 DC）的个体发育和功能的进化保守分子途径提供了独特的数据库资源。
Genome-wide expression profiling of mouse and human leukocytes reveal conserved transcriptional programs of plasmacytoid or conventional dendritic cell subsets. Dendritic cells (DCs) are a complex group of cells that play a critical role in vertebrate immunity. Lymph-node resident DCs (LN-DCs) are subdivided into conventional DC (cDC) subsets (CD11b and CD8α in mouse; BDCA1 and BDCA3 in human) and plasmacytoid DCs (pDCs). It is currently unclear if these various DC populations belong to a unique hematopoietic lineage and if the subsets identified in the mouse and human systems are evolutionary homologs. To gain novel insights into these questions, we sought conserved genetic signatures for LN-DCs and in vitro derived granulocyte-macrophage colony stimulating factor (GM-CSF) DCs through the analysis of a compendium of genome-wide expression profiles of mouse or human leukocytes. We show through clustering analysis that all LN-DC subsets form a distinct branch within the leukocyte family tree, and reveal a transcriptomal signature evolutionarily conserved in all LN-DC subsets. Moreover, we identify a large gene expression program shared between mouse and human pDCs, and smaller conserved profiles shared between mouse and human LN-cDC subsets. Importantly, most of these genes have not been previously associated with DC function and many have unknown functions. Finally, we use compendium analysis to re-evaluate the classification of interferon-producing killer DCs, lin-CD16+HLA-DR+ cells and in vitro derived GM-CSF DCs, and show that these cells are more closely linked to natural killer and myeloid cells, respectively. Our study provides a unique database resource for future investigation of the evolutionarily conserved molecular pathways governing the ontogeny and functions of leukocyte subsets, especially DCs.
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影响因子：
32.4
作者：
Fontenot, JD;Rasmussen, JP;Rudensky, AY
通讯作者：
Rudensky, AY
DOI：
10.1073/pnas.97.18.10101
发表时间：
2000-08-29
影响因子：
11.1
作者：
Alter, O;Brown, PO;Botstein, D
通讯作者：
Botstein, D
影响因子：
5.5
作者：
Chelvarajan, R. Lakshman;Liu, Yushu;Bondada, Subbarao
通讯作者：
Bondada, Subbarao
影响因子：
4.4
作者：
Chtanova, T;Newton, R;Mackay, CR
通讯作者：
Mackay, CR
影响因子：
56.9
作者：
Fogg, DK;Sibon, C;Geissmann, F
通讯作者：
Geissmann, F