Alterations of neocortical neuronal responses to acetylcholine and GABA in rats born to alcohol-dependent mothers.
Alterations of neocortical neuronal responses to acetylcholine and GABA in rats born to alcohol-dependent mothers.
复制标题
酒精依赖母亲所生大鼠新皮质神经元对乙酰胆碱和 GABA 反应的改变。
作者：
L. Janiri;G. Gobbi;A. Persico;M. Santarelli;D. Minciacchi;E. Tempesta
已知酒精是干扰神经元和突触成熟的CNS致畸因子。本研究的目的是探讨GABA能和胆碱能神经中枢机制的成年大鼠暴露于酒精在第三阶段的产前生活（ADM），当他们的母亲进行酒精身体依赖诱导（9.6 g/kg/天）。对乙酰胆碱和GABA的反应记录在额叶和体感皮层神经元。成年大鼠，其母亲已被给予安慰剂与相同的程序，被用作对照（C）。与对照组相比，ADM动物的胆碱能反应显著降低，GABA能反应增加。单次腹腔注射酒精（1.6 g/kg）后，ADM动物的自发放电受到抑制的程度低于C大鼠。C组胆碱能兴奋减少，ADM动物中阿托品拮抗作用逆转后胆碱能兴奋增强。GABA能抑制略有增加，荷包牡丹碱拮抗作用被阻断C大鼠，而ADM动物表现出减少GABA的反应。本研究结果支持了以往关于出生前和出生后酒精暴露动物的GABA能系统活性增高和胆碱能系统活性降低的研究。乙醇注射后的微离子电渗结果导致了一个假设，即快速的耐受性/依赖性可能会在酒精依赖大鼠的后代。
Alcohol is known to be a CNS teratogenic factor interfering with neuronal and synaptic maturation. The purpose of this microiontophoretic study was to explore GABAergic and cholinergic central mechanisms in adult rats exposed to alcohol in the third phase of prenatal life (ADM), when their mothers were subjected to alcohol physical dependence induction (9.6 g/kg/day). Responses to acetylcholine and GABA were recorded in frontal and somatosensory cortical neurons. Adult rats, whose mothers had been administered placebo with identical procedures, were used as a control (C). Cholinergic responses were significantly decreased and GABAergic responses increased in ADM animals with respect to controls. After a single i.p. alcohol injection (1.6 g/kg) spontaneous firing was depressed in ADM animals to a lesser extent than in C rats. Cholinergic excitations were reduced in C group and potentiated with reversal of atropine antagonism in ADM animals. GABAergic inhibitions were slightly increased and bicuculline antagonism was blocked in C rats, while ADM animals showed decreased responses to GABA. The present results support the hyperactivity of GABAergic system and the hypoactivity of cholinergic system reported in previous studies on prenatally and postnatally alcohol-exposed animals. Microiontophoretic results following ethanol injection led to the hypothesis that a rapid tolerance/dependence may develop in the offspring of alcohol-dependent rats.
产前酒精暴露对大鼠的长期行为影响。
DOI：
10.1111/j.1530-0277.1990.tb01225.x
发表时间：
1990
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Riley,EP
通讯作者：
Riley,EP