The mating type transcription factor MAT1-1-1 from the fungal human pathogen Aspergillus fumigatus: synthesis, purification, and crystallization of the DNA binding domain
The mating type transcription factor MAT1-1-1 from the fungal human pathogen Aspergillus fumigatus: synthesis, purification, and crystallization of the DNA binding domain
复制标题
来自人类病原体烟曲霉的交配型转录因子 MAT1-1-1：DNA 结合域的合成、纯化和结晶
DOI：
10.1101/2021.12.13.472399
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Hofmann E
中科院分区：
文献类型：
--
作者：
Ramšak B;Kück U;Hofmann E
交配型(MAT)基因座是子囊菌有性繁殖和发育的最重要和最重要的调节基因。通常，它们编码两个转录因子，命名为MAT1-1-1或MAT1-2-1。交配型菌株只携带两个TF基因中的一个，这两个基因控制信息素和信息素受体基因的表达，参与细胞-细胞识别过程。本文报道了人类致病真菌烟曲霉(Aspergillusfumigatus，α-1-1)的Mat1-1-1α1(AfMAT1-1)结构域的结晶。含有α-1结构域的多肽与携带AFMAT1-1-1识别序列的DNADNA之间的络合物得到了晶体。采用条纹籽晶技术提高了晶体质量，使晶体的衍射值达到3.2？分辨率。此外，从硒蛋氨酸取代的AFMAT1-1-1晶体中收集了高度冗余的数据集，最大分辨率为3.2ó。这是首次对参与子囊菌交配的α-1结构域MAT调控因子的结构进行研究。
Mating type (MAT) loci are the most important and significant regulators of sexual reproduction and development in ascomycetous fungi. Usually, they encode two transcription factors (TFs), named MAT1-1-1 or MAT1-2-1. Mating-type strains carry only one of the two TF genes, which control expression of pheromone and pheromone receptor genes, involved in the cell-cell recognition process. The present work presents the crystallization for the alpha1 (α1) domain of MAT1-1-1 from the human pathogenic fungusAspergillus fumigatus(AfMAT1-1-1). Crystals were obtained for the complex between a polypeptide containing the α1 domain and DNA carrying the AfMAT1-1-1 recognition sequence. A streak seeding technique was applied to improve native crystal quality, resulting in diffraction data to 3.2 Å resolution. Further, highly redundant data sets were collected from the crystals of selenomethionine-substituted AfMAT1-1-1 with a maximum resolution of 3.2 Å. This is the first report of structural studies on the α1 domain MAT regulator involved in the mating of ascomycetes.SynopsisAn optimized purification and crystallization protocol together with initial X-ray datasets are described for this mating type transcription factor from human pathogenic fungusAspergillus fumigatus.
影响因子：
5.6
作者：
VANDUYNE, GD;STANDAERT, RF;CLARDY, J
通讯作者：
CLARDY, J
影响因子：
11.8
作者：
van der Linden JW;Arendrup MC;Warris A;Lagrou K;Pelloux H;Hauser PM;Chryssanthou E;Mellado E;Kidd SE;Tortorano AM;Dannaoui E;Gaustad P;Baddley JW;Uekötter A;Lass-Flörl C;Klimko N;Moore CB;Denning DW;Pasqualotto AC;Kibbler C;Arikan-Akdagli S;Andes D;Meletiadis J;Naumiuk L;Nucci M;Melchers WJ;Verweij PE
通讯作者：
Verweij PE