CD36 in the periphery and brain synergizes in stroke injury in hyperlipidemia.
CD36 in the periphery and brain synergizes in stroke injury in hyperlipidemia.
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DOI：
10.1002/ana.23569
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发表时间：
2012-06
影响因子：
11.2
通讯作者：
Cho, Sunghee
中科院分区：
文献类型：
--
作者：
Kim, Eunhee;Febbraio, Maria;Bao, Yi;Tolhurst, Aaron T.;Epstein, Jeffrey M.;Cho, Sunghee
高血压加重缺血性卒中结局，并增加缺血后脑以及外周单核细胞/巨噬细胞中的CD 36表达。通过在CD 36表达和CD 36缺陷小鼠之间交换骨髓来源的细胞，本研究调查了外周CD 36与脑CD 36对高脂血症中中风病理学的贡献。在骨髓移植后，小鼠被喂食高脂肪饮食11周，然后经历缺血性中风。在缺血后3天测定中风结果、脑CD 36、MCP-1、CCR 2的表达和血浆MCP-1水平。还测定了与从CD 36表达或CD 36缺陷小鼠获得的血清孵育的脾细胞中的CD 36和CCR 2表达。从外周浸润的免疫细胞是缺血后脑中CD 36的主要来源，并有助于卒中诱导的脑损伤。这种CD 36效应依赖于外周免疫细胞中MCP-1和CCR 2表达以及宿主脑中表达CD 36的细胞的调节。这项研究表明，在外周和脑中表达的CD 36通过在高血压条件下调节MCP-1/CCR 2趋化因子轴在缺血性脑损伤中协同作用。
Hyperlipidemia exacerbates ischemic stroke outcome and increased CD36 expression in the post-ischemic brain as well as in peripheral monocytes/macrophages. By exchanging bone marrow-derived cells between CD36-expressing and –deficient mice, this study investigates the contribution of peripheral CD36 vs brain CD36 to stroke pathology in hyperlipidemia. Following bone marrow transplantation, mice were fed a high fat diet for 11 weeks, and then subjected to ischemic stroke. Stroke outcome, expression of brain CD36, MCP-1, CCR2, and plasma MCP-1 levels were determined at 3 days post-ischemia. CD36 and CCR2 expression were also determined in splenocytes incubated with serum obtained from CD36-expressing or CD36-deficient mice. Infiltrating immune cells from the periphery are the major source of CD36 in the post-ischemic brain and contribute to stroke-induced brain injury. This CD36 effect was dependent on the modulation of MCP-1 and CCR2 expression in peripheral immune cells as well as CD36-expressing cells in the host brain. This study demonstrates that CD36 expressed in the periphery and brain synergize in ischemic brain injury through regulation of the MCP-1/CCR2 chemokine axis in hyperlipidemic conditions.
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影响因子：
4.8
作者：
Cho, Sunghee;Szeto, Hazel H.;Pinto, John T.
通讯作者：
Pinto, John T.
CD36介导了对β-淀粉样蛋白的先天宿主反应。
影响因子：
15.3
作者：
El Khoury, JB;Moore, KJ;Means, TK;Leung, J;Terada, K;Toft, M;Freeman, MW;Luster, AD
通讯作者：
Luster, AD
影响因子：
6
作者：
Coraci, IS;Husemann, J;El Khoury, JB
通讯作者：
El Khoury, JB
影响因子：
15.9
作者：
Febbraio, M;Podrez, EA;Silverstein, RL
通讯作者：
Silverstein, RL
影响因子：
4.7
作者：
Cho S;Kim E
通讯作者：
Kim E