A Novel Signaling Pathway Required for Arabidopsis Endodermal Root Organization Shapes the Rhizosphere Microbiome.
A Novel Signaling Pathway Required for Arabidopsis Endodermal Root Organization Shapes the Rhizosphere Microbiome.
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DOI：
10.1093/pcp/pcaa170
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发表时间：
2021-05-11
影响因子：
4.9
通讯作者：
Gutierrez-Marcos J
中科院分区：
文献类型：
--
作者：
Durr J;Reyt G;Spaepen S;Hilton S;Meehan C;Qi W;Kamiya T;Flis P;Dickinson HG;Feher A;Shivshankar U;Pavagadhi S;Swarup S;Salt D;Bending GD;Gutierrez-Marcos J
凯氏带(CS)是植物根部水分和营养物质的物理扩散屏障，由木质素聚合物在植物内皮层组织中的极性沉积形成。木质素沉积的确切模式是由膜结合的Casparian带状结构域蛋白(CASP)的支架活性决定的，但人们对指导这一过程的机制(S)知之甚少。在这里，我们证明了内胚层特异性受体样激酶1(ERK1)和ROP结合激酶1(RBK1)也参与了CS的形成，前者在木质素沉积和CASP1的定位中起着重要作用。我们发现ERK1定位于内胚层的细胞质和胞核，并且与生物钟调节因子咖啡时间(TIC)一起，形成了一个新的信号通路的一部分，这是正确的CS组织和内皮层亚油酸化所必需的，它们的单一或联合功能丧失导致了根微生物群组成的改变。此外，我们还发现，其他突变体在根分泌物和微生物群组成上也表现出缺陷，在CS处表现出Suberin沉积的缺陷。因此，我们的工作揭示了一个复杂的信号因子网络，它作用于根内皮层，建立CS扩散屏障，并影响根际的微生物组成。
The Casparian strip (CS) constitutes a physical diffusion barrier to water and nutrients in plant roots, which is formed by the polar deposition of lignin polymer in the endodermis tissue. The precise pattern of lignin deposition is determined by the scaffolding activity of membrane-bound Casparian Strip domain proteins (CASPs), but little is known of the mechanism(s) directing this process. Here, we demonstrate that Endodermis-specific Receptor-like Kinase 1 (ERK1) and, to a lesser extent, ROP Binding Kinase1 (RBK1) are also involved in regulating CS formation, with the former playing an essential role in lignin deposition as well as in the localization of CASP1. We show that ERK1 is localized to the cytoplasm and nucleus of the endodermis and that together with the circadian clock regulator, Time for Coffee (TIC), forms part of a novel signaling pathway necessary for correct CS organization and suberization of the endodermis, with their single or combined loss of function resulting in altered root microbiome composition. In addition, we found that other mutants displaying defects in suberin deposition at the CS also display altered root exudates and microbiome composition. Thus, our work reveals a complex network of signaling factors operating within the root endodermis that establish both the CS diffusion barrier and influence the microbial composition of the rhizosphere.
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将植物营养状况与植物-微生物相互作用联系起来
影响因子：
3.7
作者：
Carvalhais LC;Dennis PG;Fan B;Fedoseyenko D;Kierul K;Becker A;von Wiren N;Borriss R
通讯作者：
Borriss R
影响因子：
64.8
作者：
Bai, Yang;Mueller, Daniel B.;Schulze-Lefert, Paul
通讯作者：
Schulze-Lefert, Paul
DOI：
10.1083/jcb.37.1.199
发表时间：
1968-04
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bonnett HT Jr
通讯作者：
Bonnett HT Jr
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF
DOI：
10.1073/pnas.0910772107
发表时间：
2010-03-16
影响因子：
11.1
作者：
Alassimone, Julien;Naseer, Sadaf;Geldner, Niko
通讯作者：
Geldner, Niko