A novel use of gentamicin in the ROS-mediated sensitization of NCI-H460 lung cancer cells to various anticancer agents.
A novel use of gentamicin in the ROS-mediated sensitization of NCI-H460 lung cancer cells to various anticancer agents.
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DOI：
10.1021/cb4007024
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发表时间：
2013-12-20
影响因子：
4
通讯作者：
O'Doherty, George A.
中科院分区：
文献类型：
--
作者：
Cuccarese, Michael F.;Singh, Amit;Amiji, Mansoor;O'Doherty, George A.
氨基糖苷类是广谱抗生素，用于治疗严重的革兰氏阴性和革兰氏阳性细菌感染。虽然氨基糖苷类的杀菌作用是由于与细菌核糖体的 30S 亚基结合所致，但氨基糖苷类可以影响真核细胞中的蛋白质合成、细胞内钙水平和活性氧 (ROS) 水平。虽然氨基糖苷类在高浓度下可能具有细胞毒性，但我们的结果表明，在低得多的剂量下，庆大霉素可以作为 NSCLC 细胞系 NCI-H460 的敏化剂，提高喜树碱、洋地黄毒苷和长春花碱的体外功效。我们还确定这种致敏依赖于庆大霉素产生的 ROS 反应。
Aminoglycosides are broad-spectrum antibiotics that are used for the treatment of severe Gram-negative and Gram-positive bacterial infections. While bactericidal effects of aminoglycosides are due to binding to the 30S subunit of the bacterial ribosome, aminoglycosides can affect protein synthesis, intracellular calcium levels and levels of reactive oxygen species (ROS) in eukaryotic cells. While aminoglycosides can be cytotoxic at high concentrations, our results show that at much lower doses, gentamicin can be implemented as a sensitizing agent for the NSCLC cell line NCI-H460, increasing the efficacy of camptothecin, digitoxin and vinblastine in vitro. We have also established that this sensitization is reliant on the ROS response generated by gentamicin.
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影响因子：
3.8
作者：
Elbaz, Hosam A.;Stueckle, Todd A.;Wang, Hua-Yu Leo;O'Doherty, George A.;Lowry, David T.;Sargent, Linda M.;Wang, Liying;Dinu, Cerasela Zoica;Rojanasakul, Yon
通讯作者：
Rojanasakul, Yon
影响因子：
7.4
作者：
Denamur, Sophie;Tyteca, Donatienne;Mingeot-Leclercq, Marie-Paule
通讯作者：
Mingeot-Leclercq, Marie-Paule
影响因子：
4.2
作者：
Wang, Hua-Yu Leo;Rojanasakul, Yon;O'Doherty, George A.
通讯作者：
O'Doherty, George A.
影响因子：
14.9
作者：
Floquet C;Deforges J;Rousset JP;Bidou L
通讯作者：
Bidou L
影响因子：
3.4
作者：
Hinds, John W.;McKenna, Sean B.;O'Doherty, George A.
通讯作者：
O'Doherty, George A.