Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C(18) column/time-of-flight mass spectrometry system.
Screening of immune cell activators from Astragali Radix using a comprehensive two-dimensional NK-92MI cell membrane chromatography/C(18) column/time-of-flight mass spectrometry system.
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DOI：
10.1016/j.jpha.2022.05.006
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发表时间：
2022-10
影响因子：
8.8
通讯作者：
Chen, Xiaofei
中科院分区：
文献类型：
--
作者：
Chai, Xinyi;Gu, Yanqiu;Lv, Lei;Chen, Chun;Feng, Fei;Cao, Yan;Liu, Yue;Zhu, Zhenyu;Hong, Zhanying;Chai, Yifeng;Chen, Xiaofei
黄芪（Astragali Radix， AR）是一种临床使用的具有多种免疫调节活性的中草药，可以增强自然杀伤细胞（NK）的活性和细胞毒性。然而，由于其组成的复杂性，AR中作用于NK细胞的具体活性成分尚不清楚。细胞膜色谱法（CMC）主要用于草药复杂体系中有效成分的筛选。在这项研究中，建立了一个新的综合二维（2D） NK- 92mi CMC/C18柱/飞行时间质谱（TOFMS）系统来筛选潜在的NK细胞激活剂。为了获得更高的柱效，合成了3-巯基丙基三甲氧基硅烷改性二氧化硅，制备了NK-92MI CMC柱。共筛选出9个AR组分，10 min后可从NK-92MI/CMC柱中洗脱，对NK-92MI/CMC柱具有良好的亲和力。AR的两种代表性活性化合物异黄芪甲苷I和黄芪甲苷IV促进NK细胞对K562细胞的杀伤作用呈剂量依赖性。由此可见，异黄芪甲苷I和黄芪甲苷IV是AR的主要免疫调节成分。该综合2D NK-92MI CMC分析系统是一种从其他具有免疫调节作用的中草药中筛选免疫细胞激活剂的实用方法。开发了一种全面的2D NK-92MI/CMC系统来筛选免疫细胞激活剂。筛选了黄芪的9种潜在免疫激活剂。异黄芪甲苷I和黄芪甲苷IV首次被证实具有免疫调节作用。
Astragali Radix (AR) is a clinically used herbal medicine with multiple immunomodulatory activities that can strengthen the activity and cytotoxicity of natural killer (NK) cells. However, owing to the complexity of its composition, the specific active ingredients in AR that act on NK cells are not clear yet. Cell membrane chromatography (CMC) is mainly used to screen the active ingredients in a complex system of herbal medicines. In this study, a new comprehensive two-dimensional (2D) NK-92MI CMC/C18 column/time-of-flight mass spectrometry (TOFMS) system was established to screen for potential NK cell activators. To obtain a higher column efficiency, 3-mercaptopropyltrimethoxysilane-modified silica was synthesized to prepare the NK-92MI CMC column. In total, nine components in AR were screened from this system, which could be washed out from the NK-92MI/CMC column after 10 min, and they showed good affinity for NK-92MI/CMC column. Two representative active compounds of AR, isoastragaloside I and astragaloside IV, promoted the killing effect of NK cells on K562 cells in a dose-dependent manner. It can thus suggest that isoastragaloside I and astragaloside IV are the main immunomodulatory components of AR. This comprehensive 2D NK-92MI CMC analytical system is a practical method for screening immune cell activators from other herbal medicines with immunomodulatory effects. A comprehensive 2D NK-92MI/CMC system was developed to screen for immune cell activators. Nine components of Astragali Radix were screened as potential immune activators. Isoastragaloside I and astragaloside IV were first confirmed to have immunomodulatory effects.
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