Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix.
Structural Characterization and Immune Activity Screening of Polysaccharides With Different Molecular Weights From Astragali Radix.
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不同分子量黄芪多糖的结构表征及免疫活性筛选
DOI：
10.3389/fphar.2020.582091
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发表时间：
2020
影响因子：
5.6
通讯作者：
Qin XM
中科院分区：
文献类型：
--
作者：
Li K;Cao YX;Jiao SM;Du GH;Du YG;Qin XM
糖份是黄芪中含量最多、免疫活性最强的物质。然而，不同分子量（Mw）的AR多糖的系统结构研究和免疫活性筛选尚未开展。本研究首次采用超滤技术将黄芪多糖（黄芪多糖）分离为3个不同Mw值的片段，分别为约2000 kDa （APS-Ⅰ）、约10 kDa （APS-Ⅱ）和约300 Da （APS-Ⅲ）。通过单糖组成、FT-IR光谱、连锁分析和核磁共振分析确定了三种产物的结构差异。采用体外细胞免疫活性实验和体内环磷酰胺免疫抑制动物模型实验进行非特异性和特异性免疫活性筛选，鉴定aps中最具免疫原性的片段。连锁分析结果表明，APS-Ⅰ、APS-Ⅱ和APS-Ⅲ具有不同的单糖残基附着位点。免疫筛选实验表明，多糖的分子量影响其活性，其中APS-Ⅱ具有最强的免疫增强活性。本研究可为进一步研究不同结构和免疫活性的APS提供参考，并为APS的质量控制和新产品的开发提供指导。
Saccharides are the most abundant substance with the strongest immunological activity in Astragali Radix (AR). However, systematic structure study and immunoactivity screening of polysaccharides with different molecular weights (Mw) in AR have yet to be conducted. In this study, Astragalus polysaccharides (APSs) were divided into three fragments of different Mw values, >2,000 kDa (APS-Ⅰ), about 10 kDa (APS-Ⅱ), and about 300 Da (APS-Ⅲ), by using ultrafiltration for the first time. The structural differences of the three products were determined on the basis of monosaccharide composition, FT-IR spectrum, linkage analysis, and nuclear magnetic resonance analysis. Cellular immune activity experiments in vitro and cyclophosphamide immunosuppression animal model experiments in vivo for nonspecific and specific immunoactivity screening were applied to identify the most immunogenic fragment in APSs. Linkage analysis results showed that APS-Ⅰ, APS-Ⅱ, and APS-Ⅲ have different attachment sites of monosaccharide residues. Immune screening experiments indicated that the Mw of the APSs influenced their activity, and APS-Ⅱ had the strongest immunoenhancing activity among the products. This research may serve as a reference for further study on APSs with different structures and immune activities, and as a guidance for the quality control of APSs and the development of new APS products.
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DOI：
10.3390/molecules23010062
发表时间：
2017-12-28
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Liu AJ;Yu J;Ji HY;Zhang HC;Zhang Y;Liu HP
通讯作者：
Liu HP
影响因子：
5.6
作者：
Du, Xiaogang;Zhao, Bing;Zeng, Xianyin
通讯作者：
Zeng, Xianyin
影响因子：
4.6
作者：
Han, Jin;Guo, Da;Gui, Bao-Song
通讯作者：
Gui, Bao-Song
影响因子：
11.2
作者：
Sun, Liqin;Wang, Ling;Zhou, Yan
通讯作者：
Zhou, Yan
DOI：
10.1016/s0002-9378(12)90907-2
发表时间：
1993-01-01
影响因子：
9.8
作者：
RUBIN, SC;FINSTAD, CL;LLOYD, KO
通讯作者：
LLOYD, KO