Milk whey culture with Propionibacterium freudenreichii ET-3 is effective on the colitis induced by 2,4,6-trinitrobenzene sulfonic acid in rats.
Milk whey culture with Propionibacterium freudenreichii ET-3 is effective on the colitis induced by 2,4,6-trinitrobenzene sulfonic acid in rats.
复制标题
乳清培养物中费氏丙酸杆菌ET-3对2,4,6-三硝基苯磺酸诱导的大鼠结肠炎有效。
DOI：
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发表时间：
2005
影响因子：
3.5
通讯作者：
O. Mogami
中科院分区：
文献类型：
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作者：
M. Uchida;O. Mogami
本研究旨在评价具有双歧活性的弗氏乳清杆菌ET-3培养乳清对2，4，6-三硝基苯磺酸(TNBS)诱导的大鼠结肠炎是否有效。为诱导结肠炎，在戊巴比妥钠麻醉下，夹闭结肠，在夹闭部分的管腔内注入0.1M TNBS。从诱导结肠炎的第二天开始，口服乳清培养物，剂量分别为1和3g/kg，每日2次，连续9天。第10天处死大鼠，测量溃疡大小。乳清培养对结肠炎的愈合有明显的促进作用，且呈剂量依赖关系，而培养基对此无明显影响。为了明确乳清中的活性物质，对乳清培养中添加丙酸和醋酸的效果进行了试验。丙酸钠能显著促进TNBS诱导的结肠炎的愈合，而醋酸钠则不能。上述结果表明，乳清培养物有可能成为治疗炎症性肠病的益生素，丙酸可能是乳清培养物中含有的活性物质之一。
This study aimed to evaluate whether milk whey culture with Propinibacterium freudenreichii ET-3 (milk whey culture), which has been reported to have Bifidogenic activity, is effective on the colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in rats. For the induction of colitis, the colon was clamped and 0.1 M TNBS in 35% ethanol was injected into the luminal side of the clamped portion under pentobarbital anesthesia. From the next day of colitis induction, milk whey culture was administered orally at doses of 1 and 3 g/kg, twice a day for 9 days. On the 10th day, rats were sacrificed and ulcer size was measured. Milk whey culture significantly accelerated the healing of the colitis in a dose-dependent manner, but culture medium did not. To clarify the active substance, the effects of propionic acid and acetic acid contained in milk whey culture was tested. Sodium propionate significantly accelerated the healing of TNBS-induced colitis, but sodium acetate did not. The above results show that milk whey culture may become a useful prebiotic for the therapy of inflammatory bowel disease and that propionic acid may be one of the active substances contained in milk whey culture.
影响因子：
3.4
作者：
KRIPKE, SA;FOX, AD;ROMBEAU, JL
通讯作者：
ROMBEAU, JL