MAML2 rearrangement in primary pulmonary mucoepidermoid carcinoma and the correlation with FLT1 expression.
MAML2 rearrangement in primary pulmonary mucoepidermoid carcinoma and the correlation with FLT1 expression.
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原发性肺粘液表皮样癌中MAML2重排及其与FLT1表达的相关性
DOI：
10.1371/journal.pone.0094399
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Jiang L
中科院分区：
文献类型：
--
作者：
Zhu F;Wang W;Hou Y;Shi J;Liu Z;He D;Bai C;Li S;Jiang L
原发性肺黏液表皮样癌是一种罕见的肿瘤，与涎腺黏液表皮样癌非常相似。后者已被证明具有t（11，19），导致MECT 1-MAML 2融合，这可能具有诊断和预后价值。然而，这种功能在PMEC的重要性还没有得到很好的研究。我们采用荧光原位杂交（FISH）技术检测42例PMEC和40例腺鳞癌（ASC）组织中MAML 2重排，并采用免疫组化（IHC）技术检测MECT 1-MAML 2潜在下游靶点包括HES 1、FLT 1和NR 4A 2的表达。然后检查临床病理学参数和患者结局。FISH分析显示50%的PMEC病例中存在MAML 2重排，这种特性在相当年轻的患者中（33岁与60岁; p = 0.001）非常突出，仅限于低级别和中等级别的病例。 免疫组化分析显示，FLT 1和HES 1在MAML 2重排组中的表达水平低于MAML 2未重排组（分别为p<0.001和p = 0.023）。 生存分析显示MAML 2重排与总生存期（p = 0.023）或无病生存期（p = 0.027）之间存在显著相关性，FLT 1与总生存期之间也存在相关性（p = 0.009）。 MAML 2重排在PMEC中出现频率较高，并且对该肿瘤具有特异性。MAML 2重排的存在和FLT 1的缺乏都倾向于赋予有利的临床结果。提示MAML 2重排和FLT 1联合检测对PMEC的诊断有重要的临床价值。
Primary pulmonary mucoepidermoid carcinoma (PMEC) is an uncommon neoplasm with remarkable resemblance to mucoepidermoid carcinoma of the salivary glands. The latter has been shown to harbor t(11,19) resulting in MECT1-MAML2 fusion, which may be of diagnostic and prognostic values. However, the importance of such feature in PMEC has not been well studied. We detected MAML2 rearrangement using fluorescence in situ hybridization (FISH) in tissue samples from 42 cases of PMEC and 40 of adenosquamous carcinoma (ASC), and the expression of potential downstream targets of MECT1-MAML2, including HES1, FLT1 and NR4A2 with immunohistochemistry (IHC). The findings were then examined regarding the clinicopathological parameters and patient outcomes. FISH analysis revealed MAML2 rearrangement in 50% of the PMEC cases, and such property was prominent in considerable younger patients (33 versus 60 years; p = 0.001) and restricted to cases of low and intermediate grades. IHC analysis showed that FLT1 and HES1 were expressed at lower level in MAML2 rearranged group than MAML2 non-rearranged group (p<0.001 and p = 0.023, respectively). Survival analysis showed significant correlation between MAML2 rearrangement and overall survival (p = 0.023) or disease-free survival (p = 0.027) as well as correlation between FLT1 and overall survival (p = 0.009). MAML2 rearrangement appears frequent in PMEC and specific with this tumor. Both the presence of MAML2 rearrangement and absence of FLT1 tend to confer a favorable clinical outcome. These findings suggest that molecular detection of MAML2 rearrangement combined with FLT1 may be of important clinical value for PMEC.
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影响因子：
11.4
作者：
Wu, LZ;Liu, JX;Griffin, JD
通讯作者：
Griffin, JD
影响因子：
51.1
作者：
Pirker, Robert;Pereira, Jose R.;O'Byrne, Kenneth J.
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O'Byrne, Kenneth J.
影响因子：
6.9
作者：
Chin, Chien-Hung;Huang, Chao-Cheng;Liu, Shih-Feng
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Liu, Shih-Feng
影响因子：
11.2
作者：
Coxon, A;Rozenblum, E;Kaye, FJ
通讯作者：
Kaye, FJ
影响因子：
2.1
作者：
Seethala, Raja R
通讯作者：
Seethala, Raja R