Subtelomeric Chromatin in the Fission Yeast S. pombe.
Subtelomeric Chromatin in the Fission Yeast S. pombe.
复制标题
裂变酵母S. pombe中的亚电体染色质。
DOI：
10.3390/microorganisms9091977
复制
发表时间：
2021-09-17
期刊：
影响因子：
4.5
通讯作者：
Partridge JF
中科院分区：
文献类型：
--
作者：
Yadav RK;Matsuda A;Lowe BR;Hiraoka Y;Partridge JF
端粒在保护基因组方面发挥着重要作用。在端粒和亚端粒结构域组装的特化抑制性染色质是这种保护作用的关键。然而，在许多生物体中，端粒和亚端粒序列的重复性质阻碍了研究工作。裂殖酵母S.由于相对容易的遗传操作和高等真核生物中重要调节蛋白的高度保守性，粟酒裂殖酵母为染色质生物学的解剖提供了重要的模型系统。端粒和端粒结合shelterin复合物是高度保守的哺乳动物，是组装的组成异染色质在亚端粒。在这篇综述中，我们试图总结最近的工作，详细介绍了不同的染色质结构内亚端粒结构域的分裂酵母的组装。这些包括异染色质SH亚端粒结构域、端粒相关序列（TAS）和组装高度浓缩的染色质簇（称为旋钮）的ST染色质结构域。具体而言，我们审查新的见解subtelomeric域的序列，不同类型的染色质组装在这些序列和组蛋白H3 K36的修改如何影响这些染色质结构。我们解决染色质结构的亚结构域之间的相互作用，以及亚端粒染色质是如何受到端粒结合的shelterin复合物和亚端粒结构域内部的常染染色质调节剂的影响。最后，我们表明，端粒聚类，这是通过凝聚的ST染色质旋钮域介导的，不依赖于这些域内的旋钮组装，但Set2，它介导H3K36甲基化。
Telomeres play important roles in safeguarding the genome. The specialized repressive chromatin that assembles at telomeres and subtelomeric domains is key to this protective role. However, in many organisms, the repetitive nature of telomeric and subtelomeric sequences has hindered research efforts. The fission yeast S. pombe has provided an important model system for dissection of chromatin biology due to the relative ease of genetic manipulation and strong conservation of important regulatory proteins with higher eukaryotes. Telomeres and the telomere-binding shelterin complex are highly conserved with mammals, as is the assembly of constitutive heterochromatin at subtelomeres. In this review, we seek to summarize recent work detailing the assembly of distinct chromatin structures within subtelomeric domains in fission yeast. These include the heterochromatic SH subtelomeric domains, the telomere-associated sequences (TAS), and ST chromatin domains that assemble highly condensed chromatin clusters called knobs. Specifically, we review new insights into the sequence of subtelomeric domains, the distinct types of chromatin that assemble on these sequences and how histone H3 K36 modifications influence these chromatin structures. We address the interplay between the subdomains of chromatin structure and how subtelomeric chromatin is influenced by both the telomere-bound shelterin complexes and by euchromatic chromatin regulators internal to the subtelomeric domain. Finally, we demonstrate that telomere clustering, which is mediated via the condensed ST chromatin knob domains, does not depend on knob assembly within these domains but on Set2, which mediates H3K36 methylation.
影响因子：
--
作者：
Chaudari A;Huberman JA
通讯作者：
Huberman JA
影响因子：
7.3
作者：
Creamer, Kevin M.;Partridge, Janet F.
通讯作者：
Partridge, Janet F.
影响因子：
14.9
作者：
Greenwood J;Patel H;Cech TR;Cooper JP
通讯作者：
Cooper JP