RITS-connecting transcription, RNA interference, and heterochromatin assembly in fission yeast.
RITS-connecting transcription, RNA interference, and heterochromatin assembly in fission yeast.
复制标题
DOI：
10.1002/wrna.80
复制
发表时间：
2011-09
影响因子：
7.3
通讯作者：
Partridge, Janet F.
中科院分区：
文献类型：
--
作者：
Creamer, Kevin M.;Partridge, Janet F.
近年来发现的大量证据表明，“沉默”的异染色质并不像以前认为的那样转录惰性。在单细胞酵母裂糖酵母（Schizosaccharomyces pombe）中，着丝粒重复序列的转录本加工成同源小干扰RNA （siRNA）是形成着丝粒异染色质所必需的。siRNA生物发生所需基因的缺失表明，典型RNAi途径的核心成分对于着丝粒异染色质组装和着丝粒功能至关重要。随后纯化的RITS （RNA诱导的转录基因沉默起始）复合物提供了sirna和异染色质组装之间的关键联系，RITS作为非编码RNA支架和染色质之间的物理桥梁。在这里，我们回顾了目前对RITS如何促进异染色质形成以及它如何参与转录偶联沉默的理解。
In recent years a bevy of evidence has been unearthed indicating that ‘silent’ heterochromatin is not as transcriptionally inert as once thought. In the unicellular yeast Schizosaccharomyces pombe, processing of transcripts derived from centromeric repeats into homologous small interfering RNA (siRNA) is essential for the formation of centromeric heterochromatin. Deletion of genes required for siRNA biogenesis revealed that core components of the canonical RNAi pathway are essential for centromeric heterochromatin assembly as well as for centromere function. Subsequent purification of the RITS (RNA-induced initiation of transcriptional gene silencing) complex provided the critical link between siRNAs and heterochromatin assembly, with RITS acting as a physical bridge between non-coding RNA scaffolds and chromatin. Here, we review current understanding of how RITS promotes heterochromatin formation and how it participates in transcription coupled silencing.
登录
查看更多内容
影响因子：
64.5
作者：
Cam HP;Chen ES;Grewal SI
通讯作者：
Grewal SI
影响因子：
64.8
作者：
Chendrimada, TP;Gregory, RI;Shiekhattar, R
通讯作者：
Shiekhattar, R
影响因子：
11.8
作者：
Emmerth, Stephan;Schober, Heiko;Buehler, Marc
通讯作者：
Buehler, Marc
影响因子：
11.4
作者：
Djupedal, Ingela;Kos-Braun, Isabelle C.;Ekwall, Karl
通讯作者：
Ekwall, Karl
影响因子：
64.5
作者：
Buhler, Marc;Verdel, André;Moazed, Danesh
通讯作者：
Moazed, Danesh