Protective effects of nicaraven, a new hydroxyl radical scavenger, on the endothelial dysfunction after exposure of pig coronary artery to hydroxyl radicals
Protective effects of nicaraven, a new hydroxyl radical scavenger, on the endothelial dysfunction after exposure of pig coronary artery to hydroxyl radicals
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新型羟自由基清除剂尼卡芬对猪冠状动脉羟自由基暴露后内皮功能障碍的保护作用
DOI：
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发表时间：
2004
影响因子：
4.3
通讯作者：
K. Tamura
中科院分区：
文献类型：
--
作者：
Mohammed Shah Alam;K. Ku;M. Yamauchi;M. Hashimoto;S. Nosaka;M. S. Hossain;S. Masumura;K. Nakayama;K. Tamura
最近，我们报道了一种新的合成化合物，1,2bis（烟酰胺）-丙烷（nicaraven），可以改善保存和再灌注后的心功能。在本研究中，我们通过体外氧化应激模型研究了尼加拉瓜文作为自由基清除剂的作用，以阐明这种新化合物对大鼠心脏再灌注损伤的保护作用机制。将猪心外膜右冠状动脉（RCA）环段悬浮于器官室中，暴露于两种不同体系中分别由0.28 mM FeSO4/0.28 mM H2O2和DHF/Fe3+-ADP（分别为2.4 mM、43 nM和1.56 uM）在沐浴液中产生的羟基自由基（·OH）中60 min。冠状动脉先前暴露于·OH显著地使缓激素诱导的内皮依赖性舒张的剂量-反应曲线向右移动（在两种不同的·OH产生系统中，缓激素的ED50值分别比对照增加4.37倍和1.98倍），但不影响最大舒张反应。nicaraven（10-4和10-5 M）在·OH生成系统中的存在，将缓激肽的剂量-反应曲线移至对照水平，表明该药物具有显著的羟基自由基清除作用。这些结果表明，尼卡拉芬作为一种新的羟基自由基清除剂，对羟基自由基诱导的猪冠状动脉内皮功能障碍具有保护作用。
Recently, we have reported that a new synthetic compound, 1,2bis(nicotinamido)-propane (nicaraven), improved cardiac function following preservation and reperfusion. In this study, we investigated the efficacy of nicaraven as a radical scavenger by using an in vitro model of oxidative stress, to clarify mechanisms of the protective effect of this new compound on reperfusion injury in rat heart. Ring segments of epicardial right coronary arteries (RCA) of pig were suspended in organ chambers and exposed to hydroxyl radicals (·OH), generated (by two different systems ) by 0.28 mM FeSO4/0.28 mM H2O2 and DHF/Fe3+-ADP (2.4 mM, 43 nM, and 1.56 uM, respectively) to the bathing solution for 60 min. Prior exposure of the coronary arteries to ·OH significantly produced right-ward shift of the dose-response curves of the bradykinin-induced endothelium-dependent relaxations (an increase in the ED50 value for bradykinin by 4.37 and 1.98 times than control in two different ·OH generating systems, respectively), but did not affect the maximum relaxation responses. The presence of nicaraven (10-4 and 10-5 M) in the ·OH generating system, shifted the dose-response curves to bradykinin to the control level, suggesting a significant hydroxyl radical scavenging effect of the drug. These results indicate that nicaraven, a new hydroxyl radical scavenger, exhibits a protective effect on hydroxyl radicalinduced endothelial dysfunctions of pig coronary artery.
猪冠状动脉内皮介导的舒张对氧和代谢的依赖性。
DOI：
10.1152/ajpheart.1993.265.1.h299
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Hashimoto,M;Close,LA;Ishida,Y;Paul,RJ
通讯作者：
Paul,RJ
犬冠状动脉再灌注损伤后内皮依赖性松弛对聚集血小板的长期损害。
影响因子：
37.8
作者：
Pearson,PJ;Schaff,HV;Vanhoutte,PM
通讯作者：
Vanhoutte,PM
DOI：
10.1073/pnas.85.11.4046
发表时间：
1988-06-01
影响因子：
11.1
作者：
ZWEIER, JL;KUPPUSAMY, P;LUTTY, GA
通讯作者：
LUTTY, GA