Long-term impairment of endothelium-dependent relaxations to aggregating platelets after reperfusion injury in canine coronary arteries.
Long-term impairment of endothelium-dependent relaxations to aggregating platelets after reperfusion injury in canine coronary arteries.
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犬冠状动脉再灌注损伤后内皮依赖性松弛对聚集血小板的长期损害。
DOI：
10.1161/01.cir.81.6.1921
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发表时间：
1990
期刊：
影响因子：
37.8
通讯作者：
Vanhoutte,PM
中科院分区：
文献类型：
--
作者：
Pearson,PJ;Schaff,HV;Vanhoutte,PM
设计并进行实验以确定冠状动脉再灌注后内皮功能是否仍然慢性受损。犬左冠状动脉前降支暴露于缺血（60分钟），然后再灌注（12周）。将再灌注动脉和正常左回旋支（对照）冠状动脉段的环（3-4 mm宽）悬浮在含有生理盐水溶液（37 ℃，用95%O2 - 5%CO2充气）的器官室中用于等长力测量。在对照组和慢性再灌注动脉中，KCl或前列腺素F2 α的内皮非依赖性收缩和一氧化氮或异丙肾上腺素的内皮非依赖性舒张相当。然而，慢性再灌注冠状动脉表现出受损的内皮依赖性舒张聚集血小板。此外，再灌注冠状动脉表现出受损的内皮依赖性舒张血小板衍生化合物腺苷二磷酸，血清素，凝血酶。然而，内皮依赖性舒张乙酰胆碱之间的控制和再灌注动脉。因此，再灌注12周后，先前闭塞的冠状动脉表现出选择性损伤的内皮依赖性舒张引起的血小板聚集。在体内，这种现象可能有利于血小板粘附、聚集和血小板诱导的冠状动脉平滑肌收缩，从而促进缺血性事件，如血管痉挛和冠状动脉血栓形成。
Experiments were designed and performed to determine whether endothelial function remained chronically impaired after coronary artery reperfusion. Canine left anterior descending coronary arteries were exposed to ischemia (60 minutes) followed by reperfusion (12 weeks). Rings (3-4 mm wide) of the reperfused artery and of normal left circumflex (control) coronary artery segments were suspended in organ chambers containing physiological saline solution (37 degrees C, gassed with 95% O2-5% CO2) for isometric force measurement. Endothelium-independent contractions to KCl or prostaglandin F2 alpha and endothelium-independent relaxations to nitric oxide or isoproterenol were comparable in control and chronically reperfused arteries. However, chronically reperfused coronary arteries exhibited impaired endothelium-dependent relaxations to aggregating platelets. In addition, the reperfused coronary arteries exhibited impaired endothelium-dependent relaxations to the platelet-derived compounds adenosine diphosphate, serotonin, and thrombin. However, the endothelium-dependent relaxations to acetylcholine were comparable between control and reperfused arteries. Thus, after 12 weeks of reperfusion, previously occluded coronary arteries exhibited a selective impairment of endothelium-dependent relaxation evoked by aggregating platelets. In vivo, this phenomenon could favor platelet adhesion, aggregation, and platelet-induced contraction of coronary smooth muscle and thus facilitate ischemic events such as vasospasm and coronary thrombosis.
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影响因子：
37.8
作者：
LAM, JYT;CHESEBRO, JH;FUSTER, V
通讯作者：
FUSTER, V
DOI：
10.1073/pnas.84.24.9265
发表时间：
1987-12-01
影响因子：
11.1
作者：
IGNARRO, LJ;BUGA, GM;CHAUDHURI, G
通讯作者：
CHAUDHURI, G
血小板、内皮和血管痉挛
影响因子：
6.7
作者：
P. Vanhoutte
通讯作者：
P. Vanhoutte
5-羟色胺可以介导冠状动脉的内皮依赖性舒张。
影响因子：
--
作者：
R. Cohen;J. Shepherd;P. Vanhoutte
通讯作者：
P. Vanhoutte
具有再生内皮的猪冠状动脉对聚集血小板和血清素的内皮依赖性反应性降低。
影响因子：
20.1
作者：
Shimokawa,H;Aarhus,LL;Vanhoutte,PM
通讯作者：
Vanhoutte,PM