Tumor cells and their crosstalk with endothelial cells in 3D spheroids.
Tumor cells and their crosstalk with endothelial cells in 3D spheroids.
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DOI：
10.1038/s41598-017-10699-y
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发表时间：
2017-09-05
影响因子：
4.6
通讯作者：
Benny O
中科院分区：
文献类型：
--
作者：
Shoval H;Karsch-Bluman A;Brill-Karniely Y;Stern T;Zamir G;Hubert A;Benny O
肿瘤微环境的再现是肿瘤实验模型发展的核心挑战。为了为药物开发和个性化癌症治疗提供可靠的工具，保持原始肿瘤中存在的关键特征至关重要。随着这一努力，三维（3D）细胞模型正在被广泛研究。球体是自组装的细胞聚集体，具有生理空间生长和细胞间相互作用的许多重要组成部分。在这项研究中，我们旨在研究肿瘤和内皮细胞（EC）在含有肿瘤细胞（TC）系或患者来源的癌细胞的杂交球体中的相互联系。优化了杂化球体的制备工艺，分析了杂化球体的形貌和组织样特征。我们的发现表明，毛细管样结构是在TC:EC球体的组装和生长过程中形成的，球体的形状和表面纹理可能是细胞外基质（ECM）中细胞空间侵入性的指示。建立肿瘤/间质球体混合模型对个体化医疗的实验方法具有重要意义，可能为预测药物效果提供一种可靠、低成本的方法。
Recapitulating the tumor microenvironment is a central challenge in the development of experimental model for cancer. To provide a reliable tool for drug development and for personalized cancer therapy, it is critical to maintain key features that exist in the original tumor. Along with this effort, 3-dimentional (3D) cellular models are being extensively studied. Spheroids are self-assembled cell aggregates that possess many important components of the physiological spatial growth and cell-cell interactions. In this study we aimed to investigate the interconnection between tumor and endothelial cells (EC) in hybrid spheroids containing either tumor cell (TC) lines or patient derived cancer cells. Preparation protocols of hybrid spheroids were optimized and their morphology and tissue-like features were analyzed. Our finding show that capillary-like structures are formed upon assembly and growth of TC:EC spheroids and that spheroids’ shape and surface texture may be an indication of spatial invasiveness of cells in the extra-cellular matrix (ECM). Establishing a model of hybrid tumor/stroma spheroids has a crucial importance in the experimental approach for personalized medicine, and may offer a reliable and low-cost method for the goal of predicting drug effects.
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影响因子：
14
作者：
Hsiao, Amy Y.;Torisawa, Yu-suke;Tung, Yi-Chung;Sud, Sudha;Taichman, Russell S.;Pienta, Kenneth J.;Takayama, Shuichi
通讯作者：
Takayama, Shuichi
影响因子：
28.2
作者：
Kar SP;Beesley J;Amin Al Olama A;Michailidou K;Tyrer J;Kote-Jarai Z;Lawrenson K;Lindstrom S;Ramus SJ;Thompson DJ;ABCTB Investigators;Kibel AS;Dansonka-Mieszkowska A;Michael A;Dieffenbach AK;Gentry-Maharaj A;Whittemore AS;Wolk A;Monteiro A;Peixoto A;Kierzek A;Cox A;Rudolph A;Gonzalez-Neira A;Wu AH;Lindblom A;Swerdlow A;AOCS Study Group & Australian Cancer Study (Ovarian Cancer);APCB BioResource;Ziogas A;Ekici AB;Burwinkel B;Karlan BY;Nordestgaard BG;Blomqvist C;Phelan C;McLean C;Pearce CL;Vachon C;Cybulski C;Slavov C;Stegmaier C;Maier C;Ambrosone CB;Høgdall CK;Teerlink CC;Kang D;Tessier DC;Schaid DJ;Stram DO;Cramer DW;Neal DE;Eccles D;Flesch-Janys D;Edwards DR;Wokozorczyk D;Levine DA;Yannoukakos D;Sawyer EJ;Bandera EV;Poole EM;Goode EL;Khusnutdinova E;Høgdall E;Song F;Bruinsma F;Heitz F;Modugno F;Hamdy FC;Wiklund F;Giles GG;Olsson H;Wildiers H;Ulmer HU;Pandha H;Risch HA;Darabi H;Salvesen HB;Nevanlinna H;Gronberg H;Brenner H;Brauch H;Anton-Culver H;Song H;Lim HY;McNeish I;Campbell I;Vergote I;Gronwald J;Lubiński J;Stanford JL;Benítez J;Doherty JA;Permuth JB;Chang-Claude J;Donovan JL;Dennis J;Schildkraut JM;Schleutker J;Hopper JL;Kupryjanczyk J;Park JY;Figueroa J;Clements JA;Knight JA;Peto J;Cunningham JM;Pow-Sang J;Batra J;Czene K;Lu KH;Herkommer K;Khaw KT;kConFab Investigators;Matsuo K;Muir K;Offitt K;Chen K;Moysich KB;Aittomäki K;Odunsi K;Kiemeney LA;Massuger LF;Fitzgerald LM;Cook LS;Cannon-Albright L;Hooning MJ;Pike MC;Bolla MK;Luedeke M;Teixeira MR;Goodman MT;Schmidt MK;Riggan M;Aly M;Rossing MA;Beckmann MW;Moisse M;Sanderson M;Southey MC;Jones M;Lush M;Hildebrandt MA;Hou MF;Schoemaker MJ;Garcia-Closas M;Bogdanova N;Rahman N;NBCS Investigators;Le ND;Orr N;Wentzensen N;Pashayan N;Peterlongo P;Guénel P;Brennan P;Paulo P;Webb PM;Broberg P;Fasching PA;Devilee P;Wang Q;Cai Q;Li Q;Kaneva R;Butzow R;Kopperud RK;Schmutzler RK;Stephenson RA;MacInnis RJ;Hoover RN;Winqvist R;Ness R;Milne RL;Travis RC;Benlloch S;Olson SH;McDonnell SK;Tworoger SS;Maia S;Berndt S;Lee SC;Teo SH;Thibodeau SN;Bojesen SE;Gapstur SM;Kjær SK;Pejovic T;Tammela TL;GENICA Network;PRACTICAL consortium;Dörk T;Brüning T;Wahlfors T;Key TJ;Edwards TL;Menon U;Hamann U;Mitev V;Kosma VM;Setiawan VW;Kristensen V;Arndt V;Vogel W;Zheng W;Sieh W;Blot WJ;Kluzniak W;Shu XO;Gao YT;Schumacher F;Freedman ML;Berchuck A;Dunning AM;Simard J;Haiman CA;Spurdle A;Sellers TA;Hunter DJ;Henderson BE;Kraft P;Chanock SJ;Couch FJ;Hall P;Gayther SA;Easton DF;Chenevix-Trench G;Eeles R;Pharoah PD;Lambrechts D
通讯作者：
Lambrechts D
影响因子：
3.7
作者：
Cheng G;Tse J;Jain RK;Munn LL
通讯作者：
Munn LL
影响因子：
46.9
作者：
Helmlinger, G;Netti, PA;Jain, RK
通讯作者：
Jain, RK
影响因子：
4.8
作者：
Heiss, Maximilian;Hellstrom, Mats;Korff, Thomas
通讯作者：
Korff, Thomas