Evidence for Hepatocyte Differentiation from Embryonic Stem Cells In Vitro
Evidence for Hepatocyte Differentiation from Embryonic Stem Cells In Vitro
复制标题
体外胚胎干细胞分化为肝细胞的证据
DOI：
10.3727/000000002783985675
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发表时间：
2002
影响因子：
3.3
通讯作者：
H. Taniguchi
中科院分区：
文献类型：
--
作者：
H. Miyashita;A. Suzuki;K. Fukao;H. Nakauchi;H. Taniguchi
我们在体外证实了胚胎干（ES）细胞的肝细胞分化。 RT-PCR 分析表明，在通过形成胚状体 (EB) 及其附着培养而分化的 ES 细胞中观察到了广泛的肝基因表达。定量PCR分析显示，通过ES细胞的体外分化，与早期和晚期肝脏发育相关的肝脏基因表达得到增强。通过免疫细胞化学分析证实了附着的 EB 周围区域存在产生白蛋白的细胞。未来的实验将揭示在体外诱导 ES 细胞分化为肝细胞的分子。这项研究将为研究肝脏发育机制提供系统，并建立基于 ES 细胞的肝脏疾病治疗方法。
We confirmed hepatocyte differentiation from embryonic stem (ES) cells in vitro. RT-PCR analysis revealed that a broad range of hepatic gene expression was observed in ES cells differentiated through formation of embryoid bodies (EBs) and its attachment culture. Quantitative PCR analysis revealed that hepatic gene expression related to early and late-stage liver development were enhanced through in vitro differentiation of ES cells. The presence of albumin-producing cells in the peripheral region of attached EBs was confirmed by immunocytochemical analysis. Future experiments will reveal the molecules that induce hepatocyte differentiation from ES cells in vitro. This research will provide systems for the investigation of mechanisms in liver development and establish a method of ES cell-based therapy for liver diseases.
影响因子：
15.9
作者：
Klug, MG;Soonpaa, MH;Field, LJ
通讯作者：
Field, LJ
影响因子：
56.9
作者：
Brüstle, O;Jones, KN;McKay, RDG
通讯作者：
McKay, RDG