An Extensive Survey of Ciguatoxins on Grouper Variola louti from the Ryukyu Islands, Japan, Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
An Extensive Survey of Ciguatoxins on Grouper Variola louti from the Ryukyu Islands, Japan, Using Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS)
复制标题
使用液相色谱-串联质谱 (LC-MS/MS) 对日本琉球群岛石斑鱼中的雪卡毒素进行广泛调查
DOI：
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发表时间：
2022
影响因子：
2.9
通讯作者：
Takeshi Yasumoto
中科院分区：
文献类型：
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作者：
Naomasa Oshiro;Hiroya Nagasawa;Miharu Watanabe;Mio Nishimura;Kyoko Kuniyoshi;Naoki Kobayashi;Y. Sugita‐Konishi;H. Asakura;K. Tachihara;Takeshi Yasumoto
食用受源自底栖甲藻的雪卡毒素污染的鱼类会导致雪卡毒中毒。CFP主要发生在热带和亚热带印度洋-太平洋地区和加勒比海。此外，它偶尔发生在日本，特别是在台湾和日本九州之间的琉球群岛。牛痘是最常见的鱼类，其毒素成分包括雪卡毒素-1B和两种脱氧同系物。因此，使用液相色谱-串联质谱法（LC-MS/MS），我们分析了来自不同地点的154个个体的肉中的CTX，并在99个样本（64%）中检测到CTX。在65条鱼（43%）中，CTX水平超过了食品和药物管理局（FDA）的指导水平（0.01微克/千克）。此外，有4份样本（3%）符合日本的指导水平（>0.18 µg/kg）。此外，尽管最高的总CTX水平为0.376 µg/kg，但假设食用180 g该标本会导致CFP。此外，仅检测到CTX 1B、52-差向-54-脱氧CTX 1B和54-脱氧CTX 1B，三种CTX 1B类似物对总毒素含量的相对贡献（分别为35 ± 7.7（SD）%、27 ± 8.1%和38 ± 5.6%）与十年前在该地区报道的相似。随后，使用来自广泛地区的许多标本证实了V. lanca毒素谱的一致性。如观察到的，总CTX水平与鱼的大小相关，包括标准长度（r = 0.503，p = 3.08 × 10−11），体重（r = 0.503，p = 3.01 × 10−11）和估计年龄（r = 0.439，p = 3.81 × 10−7）。此外，虽然在条件因子（CF）和总CTX水平之间没有观察到相关性，但是在由中值CF（3.04）分开的较瘦和较胖的鱼的组之间观察到显著差异（p = 0.039）。结果还显示，CTX水平最高（>0.18 µg/kg）的4个标本的CF介于2.49和2.87之间，它们比所有标本的平均值（3.03）和中位数更瘦。
Ingesting fish contaminated with ciguatoxins (CTXs) originating from epibenthic dinoflagellates causes ciguatera fish poisoning (CFP). CFP occurs mainly in the tropical and subtropical Indo–Pacific region and the Caribbean Sea. Furthermore, it occurs sporadically in Japan, especially in the Ryukyu Islands between Taiwan and Kyushu, Japan. Variola louti is the most frequently implicated fish with a suggested toxin profile, consisting of ciguatoxin-1B and two deoxy congeners. Therefore, using the liquid chromatography–tandem mass spectrometry (LC-MS/MS), we analyzed CTXs in the flesh of 154 individuals from various locations and detected CTXs in 99 specimens (64%). In 65 fish (43%), CTX levels exceeded the Food and Drug Administration (FDA) guidance level (0.01 µg/kg). Furthermore, in four specimens (3%), the guideline level in Japan (>0.18 µg/kg) was met. Additionally, although the highest total CTX level was 0.376 µg/kg, the consumption of 180 g of this specimen was assumed to cause CFP. Moreover, only CTX1B, 52-epi-54-deoxyCTX1B, and 54-deoxyCTX1B were detected, with the relative contribution of the three CTX1B analogs to the total toxin content (35 ± 7.7 (SD)%, 27 ± 8.1%, and 38 ± 5.6%, respectively) being similar to those reported in this region in a decade ago. Subsequently, the consistency of the toxin profile in V. louti was confirmed using many specimens from a wide area. As observed, total CTX levels were correlated with fish sizes, including standard length (r = 0.503, p = 3.08 × 10−11), body weight (r = 0.503, p = 3.01 × 10−11), and estimated age (r = 0.439, p = 3.81 × 10−7) of the specimens. Besides, although no correlation was observed between condition factor (CF) and total CTX levels, a significance difference was observed (p = 0.039) between the groups of skinnier and fattier fish, separated by the median CF (3.04). Results also showed that the CF of four specimens with the highest CTX level (>0.18 µg/kg) ranged between 2.49 and 2.87, and they were skinnier than the average (3.03) and median of all specimens.
将雪卡中毒与鱼类空间生态学联系起来：通过结合非致命性血液采样和生物遥测来检查大梭鱼生物毒素水平分布的新方法。
DOI：
10.1016/j.scitotenv.2011.11.053
发表时间：
2012
期刊：
The Science of the total environment
影响因子：
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作者：
O'Toole,AmandaC;DechraouiBottein,Marie-Yasmine;Danylchuk,AndyJ;Ramsdell,JohnS;Cooke,StevenJ
通讯作者：
Cooke,StevenJ