Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain.
Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain.
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DOI：
10.3389/fpls.2021.638181
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发表时间：
2021
影响因子：
5.6
通讯作者：
Bardor M
中科院分区：
文献类型：
--
作者：
Galas L;Burel C;Schapman D;Ropitaux M;Bernard S;Bénard M;Bardor M
三角褐指藻（Phaeodactylum tricornutum）是一种海洋单细胞微藻，有三种主要形态：椭圆形、梭形和三辐射形。前人的工作已经证明，三角褐指藻Pt 3菌株的椭圆形形态具有特定的代谢特征。在这里，我们通过透射电子显微镜和先进的光学显微镜比较了硅藻P. tricornutum Pt 3菌株的主要形态类型的细胞组织。这三种形态类型具有相似之处，包括质体的光谱特征、细胞核的位置、质体周围线粒体的组织以及F-肌动蛋白皮质和F-肌动蛋白细胞内网络的存在。相反，与梭形细胞和三辐形细胞相比，卵圆细胞自发地更迅速地释放蛋白质。此外，与梭形或三辐状细胞的卵圆形的全转录组的比较显示了许多属于复杂的动态分泌机制的阳性和阴性调节剂的差异表达。这项研究强调了卵圆形态型内发生的特异性，这些特异性是卵圆细胞分泌蛋白质更快的基础。
The diatom Phaeodactylum tricornutum is a marine unicellular microalga that exists under three main morphotypes: oval, fusiform, and triradiate. Previous works have demonstrated that the oval morphotype of P. tricornutum Pt3 strain presents specific metabolic features. Here, we compared the cellular organization of the main morphotypes of the diatom P. tricornutum Pt3 strain through transmission electron and advanced light microscopies. The three morphotypes share similarities including spectral characteristics of the plastid, the location of the nucleus, the organization of mitochondria around the plastid as well as the existence of both a F-actin cortex, and an intracellular network of F-actin. In contrast, compared to fusiform and triradiate cells, oval cells spontaneously release proteins more rapidly. In addition, comparison of whole transcriptomes of oval versus fusiform or triradiate cells revealed numerous differential expression of positive and negative regulators belonging to the complex dynamic secretory machinery. This study highlights the specificities occurring within the oval morphotype underlying that the oval cells secrete proteins more rapidly.
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影响因子：
6.4
作者：
Hempel F;Maier UG
通讯作者：
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影响因子：
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作者：
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影响因子：
13.8
作者：
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Matthijs M
DOI：
10.1007/978-3-319-27216-0_16
发表时间：
2016-01-01
期刊：
ADVANCED TECHNOLOGIES FOR PROTEIN COMPLEX PRODUCTION AND CHARACTERIZATION
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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