Analysis of malto-oligosaccharides and related metabolites in rice endosperm during development
Analysis of malto-oligosaccharides and related metabolites in rice endosperm during development
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水稻胚乳发育过程中麦芽低聚糖及相关代谢物的分析
DOI：
10.1007/s00425-020-03401-6
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发表时间：
2020
期刊：
影响因子：
4.3
通讯作者：
Hiroto Kawashima
中科院分区：
文献类型：
--
作者：
Yasunori Nakamura;Masami Ono;Momoka Suto;Hiroto Kawashima
主要conclusionLinear glucans与聚合度高达23被检测到在水稻胚乳中的胚乳发育的非常早期的阶段，并被认为在从头合成的支链glucans. Abstract的重要作用知之甚少有关的贡献，麦芽寡糖（MOS）和更长的线性葡聚糖淀粉的生物合成在谷物胚乳。本研究测定了水稻胚乳发育过程中主要代谢中间产物（聚合度分别为≤ 9和≥ 10的MOS和线性葡聚糖）含量的变化。显着量的线性葡聚糖至少DP 23存在于胚乳中授粉后3和5天（DAP），而大多数MOS的DP高达8检测到胚乳中的整个发展到20 DAP。胚乳发育过程中含有大量的蔗糖、葡萄糖、果糖等单糖和苹果酸、柠檬酸、琥珀酸等有机酸。虽然代谢产物的水平与代谢流的程度没有直接关系，但本研究结果表明，MOS和线性葡聚糖以及这些糖和有机酸参与了水稻胚乳淀粉的生物合成。直链葡聚糖可能在水稻胚乳淀粉合成中发挥作用，推测其可能是随后合成支链葡聚糖的前体，所述支链葡聚糖参与胚乳发育早期的启动过程。
Main conclusionLinear glucans with degree of polymerization of up to 23 were detected in rice endosperm at the very early developmental stage of endosperm and considered to play an important role in the de novo synthesis of branched glucans.AbstractLittle is known concerning the contribution of malto-oligosaccharides (MOS) and longer linear glucans to the starch biosynthesis in cereal endosperm. In the present study, the changes in the amount of major metabolic intermediates including MOS and linear glucans with a degree of polymerization (DP) of ≤ 9 and ≥ 10, respectively, in rice endosperm were measured during the development. Significant amounts of linear glucans of at least DP23 were present in the endosperm at 3 and 5 days after pollination (DAP), whereas most MOS of DP up to 8 were detected in the endosperm throughout the development up to 20 DAP. It was also found that a significant amount of simple sugars such as sucrose, glucose, and fructose, and organic acids such as malic acid, citric acid, and succinic acid were present in the developing endosperm. Although the levels of metabolites are not directly related to the extent of the metabolic flux, the present results suggest that MOS and linear glucans as well as these sugars and organic acids are involved in starch biosynthesis of rice endosperm. It is thought that linear glucans might play a role in starch biosynthesis in rice endosperm, presumably as the precursor for the subsequent synthesis of branched glucans involved in the initiation process that is possibly active in the endosperm at the very early developmental stage.
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