Characterization of colloidal structures during intestinal lipolysis using small-angle neutron scattering.
Characterization of colloidal structures during intestinal lipolysis using small-angle neutron scattering.
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DOI：
10.1016/j.jcis.2017.03.109
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发表时间：
2017-08-01
影响因子：
9.9
通讯作者：
Chen SH
中科院分区：
文献类型：
--
作者：
Rezhdo O;Di Maio S;Le P;Littrell KC;Carrier RL;Chen SH
胆汁胶束被认为可以调节肠道吸收，部分是通过提供化合物可以分配到的相来实现的。在消化富含脂肪的食物时，胆汁胶束的溶解能力增强。我们假设，甘油三酯的肠道消化导致胶束体积的增加，可以使用小角中子散射(SANS)在消化过程中对胶束体积进行定量监测，并且SANS可以评估消化过程中存在的每个成分对胶束大小的贡献。SANS被用来表征在体外模拟肠道消化食物相关模型脂三油油之前和期间存在的胶束的大小和形状。模拟进食条件下胆汁浓度的胆盐和磷脂的预脂解混合物被组织在胶束中，平均体积为40Nm~3。在脂肪分解过程中，胶束体积在2小时的消化期内增加了2.5倍，这是由于单甘酯和脂肪酸的插入导致胶束向一个方向生长。这些努力代表了对摄入富含脂肪的食物后肠道环境溶解能力变化的定量机制理解的基础。
Bile micelles are thought to mediate intestinal absorption, in part by providing a phase into which compounds can partition. Solubilizing capacity of bile micelles is enhanced during the digestion of fat rich food. We hypothesized that the intestinal digestion of triglycerides causes an increase in volume of micelles that can be quantitatively monitored over the course of digestion using small-angle neutron scattering (SANS), and that SANS can enable evaluation of the contribution of each of the components present during digestion to the size of micelles. SANS was used to characterize the size and shape of micelles present prior to and during the in vitro simulated intestinal digestion of a model food-associated lipid, triolein. Pre-lipolysis mixtures of a bile salt and phospholipid simulating bile concentrations in fed conditions were organized in micelles with an average volume of 40 nm3. During lipolysis, the micelle volume increased 2.5-fold over a 2-hour digestion period due to growth in one direction as a result of insertion of monoglycerides and fatty acids. These efforts represent a basis for quantitative mechanistic understanding of changes in solubilizing capacity of the intestinal milieu upon ingestion of a fat-rich meal.
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