Characterization of Cep135, a novel coiled-coil centrosomal protein involved in microtubule organization in mammalian cells.
Characterization of Cep135, a novel coiled-coil centrosomal protein involved in microtubule organization in mammalian cells.
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DOI：
10.1083/jcb.200108088
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发表时间：
2002-01-07
期刊：
影响因子：
--
通讯作者：
Kuriyama R
中科院分区：
文献类型：
--
作者：
Ohta T;Essner R;Ryu JH;Palazzo RE;Uetake Y;Kuriyama R
通过使用单克隆抗体对分离的蛤中心体，我们已经确定了一种新的135-kD的中心体蛋白（Cep 135），存在于广泛的生物体。Cep 135在整个细胞周期中位于中心体，并且定位独立于微管网络。它分布在整个中心体区域，与中心粒周围的电子致密物质有关。从CHO细胞分离的cDNA序列分析预测了具有广泛α-螺旋结构域的1，145个氨基酸残基的蛋白质。一系列缺失构建体的表达揭示了三个独立的中心体靶向结构域的存在。Cep 135的过表达导致了中心体和细胞质中独特的螺旋状颗粒的积累。虽然它们的大小，形状和数量根据蛋白质表达水平而变化，但这些螺旋由排列在6 nm空间中的平行致密线组成。改变Cep 135的蛋白质过表达和/或抑制内源性Cep 135的RNA干扰引起的分裂间期和有丝分裂纺锤体微管的解体水平。因此，Cep 135可能在哺乳动物细胞中组织微管的中心体功能中发挥重要作用。
By using monoclonal antibodies raised against isolated clam centrosomes, we have identified a novel 135-kD centrosomal protein (Cep135), present in a wide range of organisms. Cep135 is located at the centrosome throughout the cell cycle, and localization is independent of the microtubule network. It distributes throughout the centrosomal area in association with the electron-dense material surrounding centrioles. Sequence analysis of cDNA isolated from CHO cells predicted a protein of 1,145–amino acid residues with extensive α-helical domains. Expression of a series of deletion constructs revealed the presence of three independent centrosome-targeting domains. Overexpression of Cep135 resulted in the accumulation of unique whorl-like particles in both the centrosome and the cytoplasm. Although their size, shape, and number varied according to the level of protein expression, these whorls were composed of parallel dense lines arranged in a 6-nm space. Altered levels of Cep135 by protein overexpression and/or suppression of endogenous Cep135 by RNA interference caused disorganization of interphase and mitotic spindle microtubules. Thus, Cep135 may play an important role in the centrosomal function of organizing microtubules in mammalian cells.
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影响因子：
5.6
作者：
HOBOHM, U;SANDER, C
通讯作者：
SANDER, C
影响因子：
7.8
作者：
KURIYAMA, R;BORISY, GG
通讯作者：
BORISY, GG
早期果蝇胚胎中心体、纺锤体和动粒中微管相关蛋白的鉴定。
DOI：
10.1083/jcb.109.6.2977
发表时间：
1989-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kellogg DR;Field CM;Alberts BM
通讯作者：
Alberts BM
DOI：
10.1083/jcb.141.1.163
发表时间：
1998-04-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Dictenberg JB;Zimmerman W;Sparks CA;Young A;Vidair C;Zheng Y;Carrington W;Fay FS;Doxsey SJ
通讯作者：
Doxsey SJ
影响因子：
7.8
作者：
KURIYAMA, R;KOFRON, M;KHODJAKOV, A
通讯作者：
KHODJAKOV, A