Bacillus subtilis BS-15 Effectively Improves Plantaricin Production and the Regulatory Biosynthesis in Lactiplantibacillus plantarum RX-8.
Bacillus subtilis BS-15 Effectively Improves Plantaricin Production and the Regulatory Biosynthesis in Lactiplantibacillus plantarum RX-8.
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枯草芽孢杆菌 BS-15 有效提高植物乳杆菌 RX-8 中植物素的产量和调节性生物合成
DOI：
10.3389/fmicb.2021.772546
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发表时间：
2021
影响因子：
5.2
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Liu G;Nie R;Liu Y;Li X;Duan J;Hao X;Shan Y;Zhang J
Plantaricin 是由植物乳杆菌产生的广谱细菌素，具有显着的食品工业应用潜力。结果发现，当与枯草芽孢杆菌 BS-15 共培养时，植物乳杆菌 RX-8 的植物素产量得到增强。因此，本研究着手探索枯草芽孢杆菌 BS-15 如何诱导植物素的生物合成。在细菌素生产者植物乳杆菌 RX-8 中，研究了与枯草芽孢杆菌 BS-15 共培养对细胞生长、植物素产生、群体感应 (QS) 信号分子 PlnA/自诱导剂-2 (AI-2) 分泌以及植物素生物合成基因簇和 AI-2 合成相关基因表达的影响。当植物乳杆菌RX-8和枯草芽孢杆菌BS-15以1:1（106:106 CFU/ml）的接种比例在Man-Rogosa-Sharp（MRS）中共接种20小时时，获得了最大的植物素产量（2,048 AU/ml），与单一培养植物乳杆菌RX-8相比增加了32倍。此外，与单一培养相比，共培养使 PlnA 诱导活性和 AI-2 活性分别提高了 8 倍和 1.14 倍。每 4 小时（4-32 小时）生成的 RT-qPCR 结果表明，枯草芽孢杆菌 BS-15 显着改善了 plnABCD 和 plnEF 的转录，并增加了 pfs 和 luxS 的转录，即使使用 200 mM D-核糖（一种 AI-2 抑制剂）也是如此。基于上述发现，与枯草芽孢杆菌BS-15共培养作为环境刺激物可以通过PlnA介导的种内QS系统和AI-2介导的种间QS系统激活plantaricin诱导。而且，PlnA和AI-2在共培养中的诱导作用是独立的。共培养中枯草芽孢杆菌BS-15的差异蛋白质组学分析表明，细菌素诱导调节机制可能与鞭毛组装、肽聚糖生物合成、无氧呼吸、甘氨酸裂解系统或焦磷酸硫胺素生物合成有关。
Plantaricin is a broad-spectrum bacteriocin produced by Lactiplantibacillus plantarum with significant food industry application potential. It was found that the plantaricin production of L. plantarum RX-8 was enhanced when co-culturing with Bacillus subtilis BS-15. This study, therefore, set out to explore how B. subtilis BS-15 induces biosynthesis of plantaricin. The effect of co-culturing with B. subtilis BS-15 on cell growth, plantaricin production, quorum-sensing (QS) signal molecule PlnA/autoinducer-2 (AI-2) secretion, as well as plantaricin biosynthesis gene cluster and AI-2 synthesis-associated gene expression, was investigated in bacteriocin-producer L. plantarum RX-8. When L. plantarum RX-8 and B. subtilis BS-15 were co-inoculated in Man–Rogosa–Sharp (MRS) for 20 h at an inoculum ratio of 1:1 (106:106 CFU/ml), the greatest plantaricin output (2,048 AU/ml) was obtained, rising by 32-fold compared with the monoculture of L. plantarum RX-8. Additionally, co-culture increased PlnA-inducing activity and AI-2 activity by 8- and 1.14-fold, respectively, over monoculture. RT-qPCR findings generated every 4 h (4–32 h) demonstrated that B. subtilis BS-15 remarkably improved the transcription of plnABCD and plnEF, and increased pfs and luxS transcription, even when using 200 mM D-ribose, a kind of AI-2 inhibitor. Based on the above findings, co-culturing with B. subtilis BS-15 as an environmental stimulus could activate the plantaricin induction via the PlnA-mediated intraspecies QS system and the AI-2-mediated interspecies QS system. Moreover, the inducing effect of PlnA and AI-2 in co-culture was independent. Differential proteomics analysis of B. subtilis BS-15 in co-culture indicated that bacteriocin-inducing regulatory mechanism may be related to flagellar assembly, peptidoglycan biosynthesis, anaerobic respiration, glycine cleavage system, or thiamin pyrophosphate biosynthesis.
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影响因子：
5.2
作者：
Gutierrez-Cortes, Carolina;Suarez, Hector;Todorov, Svetoslav Dimitrov
通讯作者：
Todorov, Svetoslav Dimitrov
影响因子：
2.8
作者：
Larsson, JT;Rogstam, A;von Wachenfeldt, C
通讯作者：
von Wachenfeldt, C
影响因子：
3
作者：
Ryu, Eun-Ju;Sim, Jaehyun;Choi, Bong-Kyu
通讯作者：
Choi, Bong-Kyu
影响因子：
4.1
作者：
Ariana, Mehdi;Hamedi, Javad
通讯作者：
Hamedi, Javad
影响因子：
2.6
作者：
Di Cagno, Raffaella;De Angelis, Maria;Gobbetti, Marco
通讯作者：
Gobbetti, Marco