RNA sequencing reveals differential expression of mitochondrial and oxidation reduction genes in the long-lived naked mole-rat when compared to mice.
RNA sequencing reveals differential expression of mitochondrial and oxidation reduction genes in the long-lived naked mole-rat when compared to mice.
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DOI：
10.1371/journal.pone.0026729
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Church GM
中科院分区：
文献类型：
--
作者：
Yu C;Li Y;Holmes A;Szafranski K;Faulkes CG;Coen CW;Buffenstein R;Platzer M;de Magalhães JP;Church GM
裸鼹鼠 (Heterocephalus glaber) 是一种长寿、抗癌的啮齿动物，人们对识别导致其这些特征和其他独特特征的适应性产生了极大的兴趣。我们利用 RNA 测序来比较裸鼹鼠和野生小鼠之间的肝脏基因表达谱。我们的结果表明，与氧化还原和线粒体相关的基因在裸鼹鼠中以较高的相对水平表达。最大的效果是上皮细胞粘附分子 (Epcam)（一种肿瘤相关蛋白）的表达量提高了近 300 倍。同样令人感兴趣的是蛋白酶抑制剂、α2-巨球蛋白 (A2m) 和线粒体复合物 II 亚基 Sdhc，这两种与衰老相关的基因在裸鼹鼠中被发现强烈过度表达。这些结果暗示了确定衰老和癌症方面物种差异的可能候选者，特别是裸鼹鼠线粒体和氧化还原途径的复杂改变。我们的差异基因表达分析通过结合使用 Illumina/Solexa 和 454 平台进行转录组测序和组装未测序物种的转录组重叠群，消除了对参考裸鼹鼠基因组的需要。总的来说，我们的工作为研究裸鼹鼠的迷人特征提供了新的研究焦点和方法。
The naked mole-rat (Heterocephalus glaber) is a long-lived, cancer resistant rodent and there is a great interest in identifying the adaptations responsible for these and other of its unique traits. We employed RNA sequencing to compare liver gene expression profiles between naked mole-rats and wild-derived mice. Our results indicate that genes associated with oxidoreduction and mitochondria were expressed at higher relative levels in naked mole-rats. The largest effect is nearly 300-fold higher expression of epithelial cell adhesion molecule (Epcam), a tumour-associated protein. Also of interest are the protease inhibitor, alpha2-macroglobulin (A2m), and the mitochondrial complex II subunit Sdhc, both ageing-related genes found strongly over-expressed in the naked mole-rat. These results hint at possible candidates for specifying species differences in ageing and cancer, and in particular suggest complex alterations in mitochondrial and oxidation reduction pathways in the naked mole-rat. Our differential gene expression analysis obviated the need for a reference naked mole-rat genome by employing a combination of Illumina/Solexa and 454 platforms for transcriptome sequencing and assembling transcriptome contigs of the non-sequenced species. Overall, our work provides new research foci and methods for studying the naked mole-rat's fascinating characteristics.
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DOI：
10.1083/jcb.139.5.1337
发表时间：
1997-12-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Litvinov SV;Balzar M;Winter MJ;Bakker HA;Briaire-de Bruijn IH;Prins F;Fleuren GJ;Warnaar SO
通讯作者：
Warnaar SO
影响因子：
3.7
作者：
de Candia P;Blekhman R;Chabot AE;Oshlack A;Gilad Y
通讯作者：
Gilad Y
影响因子：
7.8
作者：
Harper, James M.;Leathers, Charles W.;Austad, Steven N.
通讯作者：
Austad, Steven N.
影响因子：
30.8
作者：
Blacker, D;Wilcox, MA;Tanzi, RE
通讯作者：
Tanzi, RE
影响因子：
--
作者：
Hulbert, A. J.
通讯作者：
Hulbert, A. J.