High prevalence of SARS-CoV-2 antibodies in care homes affected by COVID-19: Prospective cohort study, England.
High prevalence of SARS-CoV-2 antibodies in care homes affected by COVID-19: Prospective cohort study, England.
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DOI：
10.1016/j.eclinm.2020.100597
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发表时间：
2020-11
影响因子：
15.1
通讯作者：
Zambon M
中科院分区：
文献类型：
--
作者：
Ladhani SN;Jeffery-Smith A;Patel M;Janarthanan R;Fok J;Crawley-Boevey E;Vusirikala A;Fernandez Ruiz De Olano E;Perez MS;Tang S;Dun-Campbell K;Evans EW;Bell A;Patel B;Amin-Chowdhury Z;Aiano F;Paranthaman K;Ma T;Saavedra-Campos M;Ellis J;Chand M;Brown K;Ramsay ME;Hopkins S;Shetty N;Chow JY;Gopal R;Zambon M
我们调查了六家经历COVID-19疫情的伦敦养老院，发现居民和工作人员的SARS-CoV-2感染率很高。在这里，我们报告的后续调查，包括抗体检测在同一个护理院五个星期后。初步调查的居民和工作人员重复进行了SARS-CoV-2 RT-PCR鼻拭子和基于来自感染细胞的SARS-CoV-2天然病毒抗原和病毒中和的ELISA血液检测SARS-CoV-2抗体。在初步调查的518名居民和工作人员中，186/241（77.2%）幸存居民和208/254（81.9%）工作人员接受了血清学检测。几乎所有SARS-CoV-2 RT-PCR阳性的居民和工作人员在5周后血清学阳性，无论是有症状的（居民35/35，100%;工作人员22/22，100%）还是无症状的（居民32/33，97.0%;工作人员21/22，95.5%）。有症状但SARS-CoV-2 RT-PCR阴性的居民和工作人员的血清阳性率也很高（居民23/27，85.2%;工作人员18/21，85.7%），无症状RT-PCR阴性的人也是如此（居民61/91，67.0%;工作人员95/143，66.4%）。在118/132（89.4%）血清阳性个体中检测到中和抗体，与年龄或症状无关。10名居民（10/79重新测试，12.7%）仍然RT-PCR阳性，但具有较高的RT-PCR循环阈值; 7/10进行血清学检测，所有血清学阳性。在三名居民和一名工作人员中发现了新的感染。RT-PCR提供了SARS-CoV-2感染的点流行率，但显著低估了疫情环境中的总暴露量。在经历大规模COVID-19爆发的护理院中，大多数居民和工作人员都有中和SARS-CoV-2抗体，这与年龄或症状无关。PHE
We investigated six London care homes experiencing a COVID-19 outbreak and found high rates of SARS-CoV-2 infection among residents and staff. Here we report follow-up investigations including antibody testing in the same care homes five weeks later. Residents and staff in the initial investigation had a repeat nasal swab for SARS-CoV-2 RT-PCR and a blood test for SARS CoV-2 antibodies using ELISA based on SARS-CoV-2 native viral antigens derived from infected cells and virus neutralisation. Of the 518 residents and staff in the initial investigation, 186/241 (77.2%) surviving residents and 208/254 (81.9%) staff underwent serological testing. Almost all SARS-CoV-2 RT-PCR positive residents and staff were seropositive five weeks later, whether symptomatic (residents 35/35, 100%; staff, 22/22, 100%) or asymptomatic (residents 32/33, 97.0%; staff 21/22, 95.5%). Symptomatic but SARS-CoV-2 RT-PCR negative residents and staff also had high seropositivity rates (residents 23/27, 85.2%; staff 18/21, 85.7%), as did asymptomatic RT-PCR negative individuals (residents 61/91, 67.0%; staff 95/143, 66.4%). Neutralising antibody was detected in 118/132 (89.4%) seropositive individuals and was not associated with age or symptoms. Ten residents (10/79 re-tested, 12.7%) remained RT-PCR positive but with higher RT-PCR cycle threshold values; 7/10 had serological testing and all were seropositive. New infections were detected in three residents and one staff. RT-PCR provides a point prevalence of SARS-CoV-2 infection but significantly underestimates total exposure in outbreak settings. In care homes experiencing large COVID-19 outbreaks, most residents and staff had neutralising SARS-CoV-2 antibodies, which was not associated with age or symptoms. PHE
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影响因子：
19
作者：
Harvala, Heli;Mehew, Jennifer;Zambon, Maria
通讯作者：
Zambon, Maria
影响因子：
14.2
作者：
Lombardi, A.;Consonni, D.;Gori, A.
通讯作者：
Gori, A.
影响因子：
3.5
作者：
Beyne, Tim;Chen, Yu Long;Mennink, Bart
通讯作者：
Mennink, Bart
DOI：
10.1093/infdis/jit609
发表时间：
2014-04-01
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Agnihothram S;Gopal R;Yount BL Jr;Donaldson EF;Menachery VD;Graham RL;Scobey TD;Gralinski LE;Denison MR;Zambon M;Baric RS
通讯作者：
Baric RS
影响因子：
8.8
作者：
Korth, Johannes;Wilde, Benjamin;Herrmann, Anke
通讯作者：
Herrmann, Anke