Tear Film Cytokine Profile of Patients With the Boston Keratoprosthesis Type 1: Comparing Patients With and Without Glaucoma.
Tear Film Cytokine Profile of Patients With the Boston Keratoprosthesis Type 1: Comparing Patients With and Without Glaucoma.
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DOI：
10.1167/iovs.62.4.20
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发表时间：
2021-04-01
影响因子：
4.4
通讯作者：
Harissi-Dagher M
中科院分区：
文献类型：
--
作者：
Geoffrion D;Robert MC;Di Polo A;Koenekoop RK;Agoumi Y;Harissi-Dagher M
炎性细胞因子参与青光眼的发病机制。目的是相对于对照组比较患有和不患有青光眼的波士顿人工角膜（KPro）患者泪膜中的细胞因子水平，并将水平与临床参数关联起来。该横断面研究入组了58只眼睛（58例患者）：41只KPro青光眼眼睛，7只KPro无青光眼眼睛和10只健康对照。27种细胞因子采用多重磁珠免疫法测定。在所有KPro眼中评估眼内压（IOP）、杯盘比（CDR）、视网膜神经纤维层、视力、局部用药和房角闭合。通过非参数检验分析组间细胞因子水平，并通过斯皮尔曼检验分析与临床参数的相关性。KPro伴青光眼患者的TNF-α、IL-1β、碱性FGF和IFN-γ水平显著高于KPro不伴青光眼患者（分别为P = 0.020; 0.008; 0.043; 0.018）。KPro组具有相似的特征和局部抗生素/类固醇方案。与对照组相比，青光眼KPro患者的IL-1 Ra、IL-15、VEGF和RANTES水平显著升高（分别为P < 0.001; = 0.034; < 0.001; = 0.001）。IL-1β和IFN-γ水平与CDR（分别为r = 0.309，P = 0.039和r = 0.452，P = 0.006）和IOP（分别为r = 0.292，P = 0.047和r = 0.368，P = 0.023）呈正相关。TNF-α和FGF-basic水平与CDR呈正相关（分别为r = 0.348，P = 0.022和r = 0.344，P = 0.021）。KPro青光眼患者泪液中TNF-α、IL-1β、FGF-basic、IFN-γ升高，并与CDR和IOP相关。这些结果首次在人体中显示与鼠KPro模型中记录的TNF-α和IL-1β升高一致。眼表炎症反应可能反映了KPro青光眼的炎症过程。
Inflammatory cytokines are involved in glaucoma pathogenesis. The purpose is to compare cytokine levels in the tear film of Boston keratoprosthesis (KPro) patients with and without glaucoma, relative to controls, and correlate levels with clinical parameters. This cross-sectional study enrolled 58 eyes (58 patients): 41 KPro eyes with glaucoma, 7 KPro eyes without glaucoma, and 10 healthy controls. Twenty-seven cytokines were measured by multiplex bead immunoassay. Intraocular pressure (IOP), cup-to-disk ratio (CDR), retinal nerve fiber layer, visual acuity, topical medications, and angle closure were assessed in all KPro eyes. Cytokine levels between groups were analyzed by nonparametric tests, and correlations with clinical parameters by Spearman's test. Levels of TNF-ɑ, IL-1β, FGF-basic, and IFN-ɣ were significantly higher in KPro with glaucoma compared to KPro without (P = 0.020; 0.008; 0.043; 0.018, respectively). KPro groups had similar characteristics and topical antibiotic/steroid regimen. Levels of IL-1Ra, IL-15, VEGF, and RANTES were significantly higher in KPro with glaucoma compared to controls (P < 0.001; = 0.034; < 0.001; = 0.001, respectively). IL-1β and IFN-ɣ levels were positively correlated with CDR (r = 0.309, P = 0.039 and r = 0.452, P = 0.006, respectively) and IOP (r = 0.292, P = 0.047 and r = 0.368, P = 0.023, respectively). TNF-α and FGF-basic levels were positively correlated with CDR (r = 0.348, P = 0.022 and r = 0.344, P = 0.021, respectively). TNF-α, IL-1β, FGF-basic, IFN-ɣ are elevated in tears of KPro patients with glaucoma and correlate with CDR and IOP. These results show, for the first time in humans, concordance with documented elevations of TNF-α and IL-1β in the murine KPro model. Ocular surface inflammation may reflect inflammatory processes of KPro glaucoma.
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影响因子：
13.7
作者：
Birt, CM;Shin, DH;Lee, D
通讯作者：
Lee, D
干扰素-γ 通过 Janus 激酶和 STAT3 激活抑制肠上皮水通道蛋白-1 表达。
影响因子：
3.7
作者：
Dicay MS;Hirota CL;Ronaghan NJ;Peplowski MA;Zaheer RS;Carati CA;MacNaughton WK
通讯作者：
MacNaughton WK
影响因子：
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作者：
Dohlman, Claes H.;Zhou, Chengxin;Paschalis, Eleftherios, I
通讯作者：
Paschalis, Eleftherios, I
影响因子：
3.7
作者：
Blanco, Rosa E.;Vega-Melendez, Giam S.;Blagburn, Jonathan M.
通讯作者：
Blagburn, Jonathan M.
影响因子：
4.4
作者：
Crnej, Alja;Omoto, Masahiro;Dana, Reza
通讯作者：
Dana, Reza