Repair of individual DNA strands in the hamster dihydrofolate reductase gene after treatment with ultraviolet light, alkylating agents, and cisplatin.
Repair of individual DNA strands in the hamster dihydrofolate reductase gene after treatment with ultraviolet light, alkylating agents, and cisplatin.
复制标题
用紫外线、烷化剂和顺铂处理后仓鼠二氢叶酸还原酶基因中各个 DNA 链的修复。
DOI：
10.1016/s0021-9258(18)53902-8
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
V. Bohr
中科院分区：
文献类型：
--
作者：
A. May;Rodney;S.;NairnJ;D. Okumoto;Karsten Wassermann;T. Stevnsner;Jennifer C. Jones;V. Bohr
我们分析了仓鼠细胞中二氢叶酸还原酶基因的基因特异性和链特异性DNA损伤和修复。将细胞进行UV照射或用两种类型的化疗剂（烷化剂或顺铂）处理。紫外线诱导的嘧啶二聚体检测使用先前公布的技术，其中的T4核酸内切酶V酶是用来创建在病变部位的切口。6-4在用光裂合酶预先逆转嘧啶二聚体之后，在类似的测定中使用ABC核酸内切酶检测光产物。通过中性脱嘌呤后在碱性位点产生链断裂来定量由烷化剂氮芥和硫酸二甲酯形成的加合物。顺铂诱导的链内加合物与ABC核酸内切酶检测，顺铂链间交联检测使用变性再退火反应电泳前。在雅阁与其他研究人员以前的报告，我们发现不同的链特异性的嘧啶二聚体的修复后，紫外线，转录链比非转录链更有效地修复。相比之下，在6-4光产物的修复中几乎没有或没有链偏差。对于烷化剂，在用氮芥处理后发现转录链中的修复略有偏向，但在用硫酸二甲酯处理后的修复中似乎没有偏向。顺铂链间交联从两条链以相同的效率修复，但更常见的顺铂诱导的损伤，链内加合物，优先从转录链修复。总之，在嘧啶二聚体和顺铂链内加合物的修复中存在链偏差，但修复的链特异性可能不是所有DNA损伤的一般特征，因为我们发现在研究的其他损伤的修复中很少或没有链偏差。
We have analyzed gene-specific and strand-specific DNA damage and repair in the dihydrofolate reductase gene in hamster cells. Cells were UV-irradiated or treated with two types of chemotherapeutics, alkylating agents or cisplatin. UV-induced pyrimidine dimers were detected using a previously published technique in which the T4 endonuclease V enzyme is used to create nicks at the lesion sites. 6-4 photoproducts were detected in a similar assay using ABC excinuclease after prior reversal of the pyrimidine dimers with photolyase. Adducts formed by the alkylating agents nitrogen mustard and dimethyl sulfate were quantitated by generating strand breaks at basic sites after neutral depurination. Cisplatin-induced intrastrand adducts were detected with ABC excinuclease, and cisplatin interstrand cross-links were detected using a denaturation-reannealing reaction before electrophoresis. In accord with previous reports by other investigators, we find distinct strand specificity of the repair of pyrimidine dimers after UV; the transcribed strand was much more efficiently repaired than the nontranscribed strand. In contrast, there was little or no strand bias in the repair of the 6-4 photoproducts. For alkylating agents, a slight bias toward repair in the transcribed strand was found after treatment with nitrogen mustard, but there appeared to be no bias in the repair after treatment with dimethyl sulfate. Cisplatin interstrand cross-links are repaired with equal efficiency from the two strands, but the more common cisplatin-induced lesion, the intrastrand adduct, is preferentially repaired from the transcribed strand. In conclusion, there is strand bias in the repair of pyrimidine dimers and cisplatin intrastrand adducts, but the strand specificity of repair may not be a general feature for all DNA lesions, as we found little or no strand bias in the repair of other lesions studied.
登录
查看更多内容
DOI：
--
发表时间：
1989-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. C. Thomas;D. Okumoto;A. Sancar;V. Bohr
通讯作者：
D. C. Thomas;D. Okumoto;A. Sancar;V. Bohr
定量特定哺乳动物基因中碱基加合物的通用方法。
DOI：
10.1073/pnas.85.11.3723
发表时间：
1988
影响因子：
11.1
作者：
Thomas,DC;Morton,AG;Bohr,VA;Sancar,A
通讯作者：
Sancar,A
活性胰岛素基因内碱不稳定位点的优先 DNA 修复。
DOI：
--
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
LeDoux,SP;Patton,NJ;Nelson,JW;Bohr,WA;Wilson,GL
通讯作者：
Wilson,GL
金属硫蛋白基因中的 DNA 修复随着转录激活而增加。
影响因子：
14.9
作者：
Okumoto,DS;Bohr,VA
通讯作者：
Bohr,VA
DOI：
10.1016/s0021-9258(17)39046-4
发表时间：
1985-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Photoproducts;Douglas E. Brash;William A. FranklinQll;Gwendolyn B. Sancarll;Aziz Sancarll;William A. Haseltinet
通讯作者：
Photoproducts;Douglas E. Brash;William A. FranklinQll;Gwendolyn B. Sancarll;Aziz Sancarll;William A. Haseltinet