Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft.
Influenza A Virus Hemagglutinin is Required for the Assembly of Viral Components Including Bundled vRNPs at the Lipid Raft.
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DOI：
10.3390/v8090249
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发表时间：
2016-09-10
期刊：
影响因子：
--
通讯作者：
Nomoto A
中科院分区：
文献类型：
--
作者：
Takizawa N;Momose F;Morikawa Y;Nomoto A
与脂质筏相关的流感糖蛋白、血凝素（HA）和神经氨酸酶（NA）具有启动病毒粒子出芽的潜力。然而，这些病毒蛋白在感染性病毒粒子组装中的作用尚不清楚。此外，病毒核糖核蛋白复合体（vRNP）是如何被拴在出芽位点的尚不清楚。在这里，我们证明了HA是有效产生子代病毒粒子和在病毒粒子中包装vRNP所必需的。我们还发现HA的水平不影响八个vRNP片段的捆绑，尽管减少了病毒粒子的产生。洗涤剂增溶和随后的膜浮选分析表明，在没有HA的情况下，核蛋白、病毒聚合酶、NA和基质蛋白1 （M1）在脂筏组分中的积累被推迟。基于我们的结果，我们推断HA在脂质筏上的病毒成分（包括捆绑的vRNPs）的积累中起作用。
The influenza glycoproteins, hemagglutinin (HA) and neuraminidase (NA), which are associated with the lipid raft, have the potential to initiate virion budding. However, the role of these viral proteins in infectious virion assembly is still unclear. In addition, it is not known how the viral ribonucleoprotein complex (vRNP) is tethered to the budding site. Here, we show that HA is necessary for the efficient progeny virion production and vRNP packaging in the virion. We also found that the level of HA does not affect the bundling of the eight vRNP segments, despite reduced virion production. Detergent solubilization and a subsequent membrane flotation analysis indicated that the accumulation of nucleoprotein, viral polymerases, NA, and matrix protein 1 (M1) in the lipid raft fraction was delayed without HA. Based on our results, we inferred that HA plays a role in the accumulation of viral components, including bundled vRNPs, at the lipid raft.
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