The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis.
The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis.
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DOI：
10.1186/s12865-018-0257-9
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发表时间：
2018-06-18
期刊：
影响因子：
3
通讯作者：
Razmara E
中科院分区：
文献类型：
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作者：
Sabzevary-Ghahfarokhi M;Shohan M;Shirzad H;Rahimian G;Bagheri N;Soltani A;Deris F;Ghatreh-Samani M;Razmara E
Fosl 1属于激活蛋白1（AP-1）家族，在氧化应激条件下诱导IL-11的表达。IL-11在保护上皮屏障完整性中起关键作用。在这项研究中，我们研究了Fra-1基因在溃疡性结肠炎（UC）患者炎症粘膜中的表达及其与IL-11表达的关系。我们根据临床标准招募了20例确诊为UC的患者和20例健康对照。通过实时聚合酶链反应（RT-PCR）测定炎症和非炎症结肠活检组织中的Fra-1基因表达。采用酶联免疫吸附法（ELISA）测定IL-11蛋白浓度。应用Pearson相关性计算Fra-1和IL-11之间的关系。在轻度溃疡性结肠炎患者中观察到Fra-1基因表达水平升高。轻度UC患者IL-11蛋白浓度也升高。相反，与对照组相比，在重度UC患者中检测到IL-11蛋白水平显著降低。炎症肠活检组织中的氧化应激可诱导fra-1基因表达。我们的研究结果表明，Fra-1转录因子导致UC患者IL-11蛋白的产生。
Fra-1 (fosl1) belongs to the activator protein1 (AP-1) family inducing IL-11 expression in oxidative stress condition. IL-11 plays a pivotal role in protecting epithelial barriers integrity. In this study, we investigated the Fra-1 gene expression in the inflamed mucosa of patients with ulcerative colitis (UC) as well as its relation to IL-11 expression. We enrolled 20 patients and 20 healthy controls with definite UC based on the clinical criteria. Fra-1 gene expression in inflamed and non-inflamed colonic biopsies was determined by real-time polymerase chain reaction (RT-PCR). The IL-11 protein concentration was measured by Enzyme-Linked Immunosorbent Assay (ELISA) method. Pearson correlation was applied to calculate the relation between Fra-1 and IL-11. An increased level of Fra-1 gene expression was observed in patients with mild ulcerative colitis. The protein concentration of IL-11 was also increased in mild UC patients. Conversely, a significant decrease of IL-11 protein level was detected in severe UC patients compared to control group. Oxidative stress in inflamed intestinal biopsies can induce fra-1 gene expression. Our findings suggest that Fra-1 transcription factor leads to the production of IL-11 protein in UC patients.
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影响因子：
2.3
作者：
Bozza, M;Bliss, JL;Trepicchio, WL
通讯作者：
Trepicchio, WL
影响因子：
24.5
作者：
Satsangi, J.;Silverberg, M. S.;Colombel, J-F
通讯作者：
Colombel, J-F
影响因子：
29.4
作者：
Ropeleski, MJ;Tang, J;Chang, EB
通讯作者：
Chang, EB
影响因子：
50.3
作者：
Bollrath, Julia;Phesse, Toby J.;Greten, Florian R.
通讯作者：
Greten, Florian R.
影响因子：
24.1
作者：
通讯作者：
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