A fiber-optic fluorescence microscope using a consumer-grade digital camera for in vivo cellular imaging.
A fiber-optic fluorescence microscope using a consumer-grade digital camera for in vivo cellular imaging.
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DOI：
10.1371/journal.pone.0011218
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发表时间：
2010-06-23
期刊：
影响因子：
3.7
通讯作者：
Richards-Kortum RR
中科院分区：
文献类型：
--
作者：
Shin D;Pierce MC;Gillenwater AM;Williams MD;Richards-Kortum RR
早期发现是癌症管理的重要组成部分。不幸的是，目视检查往往不可靠，而且许多环境缺乏运营PET、CT和MRI系统的资金和基础设施。此外，与外科活组织检查和显微镜检查相关的基础设施和费用是在低资源环境下建立癌症筛查/早期检测计划的挑战。性能的提高和成本的下降导致了光电子元件的出现，这些元件可以用来开发低成本的诊断成像设备，供护理地点使用。在这里，我们展示了一种使用消费级相机进行活体细胞成像的光纤荧光显微镜。该光纤荧光显微镜包括LED灯、物镜、光纤束和消费级数码相机。该系统被用来对标记了0.01%前列腺素的口腔癌细胞株进行成像。手术切除后的人体组织样本被成像，从而能够对发育不良和癌变区域进行评估。一名健康受试者的口腔粘膜在外用0.01%的前列腺素后被活体成像。光纤显微镜可以分辨出所有样本和成像组织中的单个细胞核。这一能力使得能够识别正常组织和癌前病变或癌组织之间的定性和定量差异。该系统的光学效率允许对人体口腔粘膜进行实时成像。我们的结果表明，这种装置是一种有用的工具，有助于识别上皮组织的早期肿瘤性变化。这种便携、廉价的设备可能特别适合在低资源环境中的护理点使用。
Early detection is an essential component of cancer management. Unfortunately, visual examination can often be unreliable, and many settings lack the financial capital and infrastructure to operate PET, CT, and MRI systems. Moreover, the infrastructure and expense associated with surgical biopsy and microscopy are a challenge to establishing cancer screening/early detection programs in low-resource settings. Improvements in performance and declining costs have led to the availability of optoelectronic components, which can be used to develop low-cost diagnostic imaging devices for use at the point-of-care. Here, we demonstrate a fiber-optic fluorescence microscope using a consumer-grade camera for in vivo cellular imaging. The fiber-optic fluorescence microscope includes an LED light, an objective lens, a fiber-optic bundle, and a consumer-grade digital camera. The system was used to image an oral cancer cell line labeled with 0.01% proflavine. A human tissue specimen was imaged following surgical resection, enabling dysplastic and cancerous regions to be evaluated. The oral mucosa of a healthy human subject was imaged in vivo, following topical application of 0.01% proflavine. The fiber-optic microscope resolved individual nuclei in all specimens and tissues imaged. This capability allowed qualitative and quantitative differences between normal and precancerous or cancerous tissues to be identified. The optical efficiency of the system permitted imaging of the human oral mucosa in real time. Our results indicate this device as a useful tool to assist in the identification of early neoplastic changes in epithelial tissues. This portable, inexpensive unit may be particularly appropriate for use at the point-of-care in low-resource settings.
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影响因子：
4.7
作者：
Ebner, Christoph;Wurm, Elisabeth M. T.;Soyer, H. Peter
通讯作者：
Soyer, H. Peter
DOI：
10.1016/0002-9378(78)90713-5
发表时间：
1978-01-01
影响因子：
9.8
作者：
KAUFMAN, RH;ADAM, E;YOUNG, RL
通讯作者：
YOUNG, RL
影响因子：
1.6
作者：
Dromard, Tanguy;Ravaine, Valerie;Sojic, Neso
通讯作者：
Sojic, Neso
影响因子：
8
作者：
Janssen, PA;Selwood, BL;Thiessen, PN
通讯作者：
Thiessen, PN
影响因子：
3.5
作者：
Collier, Tom;Guillaud, Martial;Richards-Kortum, Rebecca
通讯作者：
Richards-Kortum, Rebecca