Sequencing and characterization of the FVB/NJ mouse genome.
Sequencing and characterization of the FVB/NJ mouse genome.
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DOI：
10.1186/gb-2012-13-8-r72
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发表时间：
2012-08-23
期刊：
影响因子：
12.3
通讯作者：
Keane TM
中科院分区：
文献类型：
--
作者：
Wong K;Bumpstead S;Van Der Weyden L;Reinholdt LG;Wilming LG;Adams DJ;Keane TM
FVB/NJ小鼠品系起源于1935年在美国国立卫生研究院建立的一个瑞士杂交鼠群体。来自该来源的小鼠被选择性地培育成对组胺二磷酸和Friend白血病病毒B株的敏感性。这导致了FVB/N近交系的建立，该品系随后被进口到杰克逊实验室并指定为FVB/NJ。FVB/NJ小鼠具有几个明显的特征，如大的原核形态，旺盛的繁殖性能和持续的大窝数，这使得它非常适合于转基因品系的生产和通用。利用下一代测序技术，我们已经对FVB/NJ的基因组进行了大约50倍的测序，并生成了相对于参考C57BL/6J基因组的单核苷酸多态、小插入/缺失多态和结构变异的全面目录。我们检测了10号染色体上先前发现的动脉粥样硬化易感性的数量性状基因座，并确定了几个先前未知的候选因果变异。FVB/NJ基因组的测序和该目录的生成使FVB/NJ中已知的变异位点的数量增加了四倍，并将有助于加速识别导致在这种广泛使用的菌株中观察到的表型的精确分子变异。
The FVB/NJ mouse strain has its origins in a colony of outbred Swiss mice established in 1935 at the National Institutes of Health. Mice derived from this source were selectively bred for sensitivity to histamine diphosphate and the B strain of Friend leukemia virus. This led to the establishment of the FVB/N inbred strain, which was subsequently imported to the Jackson Laboratory and designated FVB/NJ. The FVB/NJ mouse has several distinct characteristics, such as large pronuclear morphology, vigorous reproductive performance, and consistently large litters that make it highly desirable for transgenic strain production and general purpose use. Using next-generation sequencing technology, we have sequenced the genome of FVB/NJ to approximately 50-fold coverage, and have generated a comprehensive catalog of single nucleotide polymorphisms, small insertion/deletion polymorphisms, and structural variants, relative to the reference C57BL/6J genome. We have examined a previously identified quantitative trait locus for atherosclerosis susceptibility on chromosome 10 and identify several previously unknown candidate causal variants. The sequencing of the FVB/NJ genome and generation of this catalog has increased the number of known variant sites in FVB/NJ by a factor of four, and will help accelerate the identification of the precise molecular variants that are responsible for phenotypes observed in this widely used strain.
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影响因子：
4.4
作者：
Ren, Ru-Jing;Wang, Li-Ling;Chen, Sheng-Di
通讯作者：
Chen, Sheng-Di
影响因子：
5.8
作者：
McLaren, William;Pritchard, Bethan;Cunningham, Fiona
通讯作者：
Cunningham, Fiona
影响因子：
5.8
作者：
Li, Heng
通讯作者：
Li, Heng
影响因子：
4.5
作者：
Naj AC;Beecham GW;Martin ER;Gallins PJ;Powell EH;Konidari I;Whitehead PL;Cai G;Haroutunian V;Scott WK;Vance JM;Slifer MA;Gwirtsman HE;Gilbert JR;Haines JL;Buxbaum JD;Pericak-Vance MA
通讯作者：
Pericak-Vance MA
影响因子：
3.7
作者：
Saade S;Cazier JB;Ghassibe-Sabbagh M;Youhanna S;Badro DA;Kamatani Y;Hager J;Yeretzian JS;El-Khazen G;Haber M;Salloum AK;Douaihy B;Othman R;Shasha N;Kabbani S;Bayeh HE;Chammas E;Farrall M;Gauguier D;Platt DE;Zalloua PA;FGENTCARD consortium
通讯作者：
FGENTCARD consortium