Berberine ameliorates nonalcoholic fatty liver disease by decreasing the liver lipid content via reversing the abnormal expression of MTTP and LDLR.
Berberine ameliorates nonalcoholic fatty liver disease by decreasing the liver lipid content via reversing the abnormal expression of MTTP and LDLR.
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DOI：
10.3892/etm.2021.10543
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发表时间：
2021-10
影响因子：
2.7
通讯作者：
Xiao L
中科院分区：
文献类型：
--
作者：
Chen P;Li Y;Xiao L
非酒精性脂肪性肝病（NAFLD）的全球发病率正在增加。本研究旨在探讨小檗碱（BBR）对大鼠非酒精性脂肪肝（NAFLD）的治疗作用及其机制。将35只SD大鼠随机分为对照组和NAFLD组，分别给予正常饲料和高脂饲料喂养8周。对肝组织进行苏木精和伊红染色，并通过显微镜证实NAFLD模型的建立。随后将NAFLD大鼠随机细分并用盐水或BBR处理8周。测量各组大鼠肝脏湿重，光镜观察肝组织结构，半自动生化检测仪检测丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总胆固醇（TC）、甘油三酯（TG）、空腹血糖（FBG）、低密度脂蛋白（LDL）、高密度脂蛋白（HDL）水平。采用逆转录-定量PCR和蛋白质印迹法检测肝微粒体甘油三酯转运蛋白（MTTP）、载脂蛋白B和低密度脂蛋白受体（LDLR）的mRNA和蛋白表达水平。与对照组相比，NAFLD大鼠肝脏湿重增加，肝脏出现明显脂肪变性，肝脏TG水平显著升高，血清ALT、AST、TC、TG、FBG、HDL、LDL水平显著升高，MTTP和LDLR表达显著降低。与生理盐水处理的NAFLD大鼠相比，BBR处理的大鼠肝脏湿重减少，肝脏脂肪变性改善，肝脏TG水平显著降低，而ALT，AST，TC，TG和LDL血清水平显著降低，MTTP水平显著上调。总之，BBR治疗改善了高脂饮食诱导的大鼠脂肪肝。BBR可逆转高脂饮食诱导的NAFLD大鼠MTTP和LDLR的异常表达。提示BBR可通过逆转MTTP和LDLR的异常表达，抑制脂质合成，从而改善脂肪肝。
The global incidence of nonalcoholic fatty liver disease (NAFLD) is increasing. The present study explored the effect and mechanism of berberine (BBR) on NAFLD in rats. Thirty-five Sprague-Dawley rats were randomly divided into the control and NAFLD groups, which were fed a normal diet or high-fat diet, respectively, for 8 weeks. Hematoxylin and eosin staining was performed on liver tissues and establishment of the NAFLD model was confirmed by microscopy. NAFLD rats were subsequently randomly subdivided and treated with saline or BBR for 8 weeks. The liver wet weight of rats in each group was measured, the liver tissue structure was observed by microscopy, and alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), fasting blood glucose (FBG), low-density lipoprotein (LDL) and high-density lipoprotein (HDL) levels were detected using a semi-automatic biochemical detector. Reverse transcription-quantitative PCR and western blotting were performed to determine the mRNA and protein expression levels of microsomal triglyceride transfer protein (MTTP), apolipoprotein B and low-density lipoprotein receptor (LDLR). Compared with the control group, the liver wet weight of the NAFLD rats was higher; the liver showed obvious fatty degeneration and liver TG levels increased significantly, as did serum levels of ALT, AST, TC, TG, FBG, HDL and LDL, while expression of MTTP and LDLR significantly decreased. Compared with the saline-treated NAFLD rats, the BBR-treated rats had reduced liver wet weight, improved liver steatosis and a significant decrease in liver TG levels, while ALT, AST, TC, TG, and LDL serum levels significantly decreased and MTTP levels were significantly upregulated. In conclusion, BBR treatment ameliorated the fatty liver induced by a high-fat diet in rats. Furthermore, BBR reversed the abnormal expression of MTTP and LDLR in rats with high-fat diet induced-NAFLD. The present findings suggest that fatty liver could be improved by BBR administration, via reversing the abnormal expression of MTTP and LDLR and inhibiting lipid synthesis.
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DOI：
10.1152/ajpendo.90710.2008
发表时间：
2009-04-01
影响因子：
5.1
作者：
Kim, Woo Sik;Lee, Yun Sok;Kim, Jae Bum
通讯作者：
Kim, Jae Bum
影响因子：
3.9
作者：
Gibbons, GF;Wiggins, D;Hebbachi, AM
通讯作者：
Hebbachi, AM
影响因子：
6.5
作者：
Brusq, Jean-Marie;Ancellin, Nicolas;Issandou, Marc
通讯作者：
Issandou, Marc
影响因子：
8.2
作者：
Forbes, S.;Taylor-Robinson, S. D.;Johnston, D. G.
通讯作者：
Johnston, D. G.
影响因子：
13.5
作者：
Browning, JD;Szczepaniak, LS;Hobbs, HH
通讯作者：
Hobbs, HH