Generation of a transgenic medaka (Oryzias latipes) strain for visualization of nuclear dynamics in early developmental stages
Generation of a transgenic medaka (Oryzias latipes) strain for visualization of nuclear dynamics in early developmental stages
复制标题
转基因青鳉 (Oryzias latipes) 菌株的生成，用于早期发育阶段核动力学的可视化
DOI：
10.1111/dgd.12324
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Kinoshita M
中科院分区：
文献类型：
--
作者：
Inoue T;Iida A;Maegawa S;Sehara-Fujisawa A;Kinoshita M
在这项研究中，我们验证了人工核定位信号(ANLS)在青冈鱼(Oryzias Latipe)体内的核运输活性。我们产生了一个转基因青竹菌株，表达由青竹β-肌动蛋白启动子驱动的aNLS标记的增强型绿色荧光蛋白(EGFP)。ANLS-EGFP在卵母细胞的体细胞核和卵黄核中均有表达，但在精子中检测不到。荧光信号是从受精后立即观察到的，这是由母亲贡献的。此外，受精卵中显示了雄性和雌性原核，并通过时间推移成像捕捉到了原核融合和随后的卵裂的核动态。相反，SV40NLS在早期胚胎中没有表现出核转运活性。综上所述，aNLS具有很强的核定位活性，是青竹早期发育阶段原核和核的荧光观察的有用探针。
In this study, we verified nuclear transport activity of an artificial nuclear localization signal (aNLS) in medaka fish (Oryzias latipes). We generated a transgenic medaka strain expresses the aNLS tagged enhanced green fluorescent protein (EGFP) driven by a medaka beta‐actin promoter. The aNLS‐EGFP was accumulated in the nuclei of somatic tissues and yolk nuclei of oocytes, but undetectable in the spermatozoa. The fluorescent signal was observed from immediately after fertilization by a maternal contribution. Furthermore, male and female pronuclei were visualized in fertilized eggs, and nuclear dynamics of pronuclear fusion and subsequent cleavage were captured by time‐lapse imaging. In contrast, SV40NLS exhibited no activity of nuclear transport in early embryos. In conclusion, the aNLS possesses a strong nuclear localization activity and is a useful probe for fluorescent observation of the pronuclei and nuclei in early developmental stage of medaka.
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遗传物质的融合
DOI：
--
发表时间：
2000
期刊：
--
影响因子：
--
作者：
S. Gilbert
通讯作者：
S. Gilbert
影响因子：
0.9
作者：
Iwai, Toshiharu;Inoue, Shinya;Yamashita, Masakane
通讯作者：
Yamashita, Masakane
影响因子：
19
作者：
D. Goldfarb;A. Corbett;D. A. Mason;Michelle T. Harreman;S. Adam
通讯作者：
D. Goldfarb;A. Corbett;D. A. Mason;Michelle T. Harreman;S. Adam
DOI：
10.1073/pnas.0900604106
发表时间：
2009-06-23
影响因子：
11.1
作者：
Kosugi, Shunichi;Hasebe, Masako;Yanagawa, Hiroshi
通讯作者：
Yanagawa, Hiroshi
影响因子：
3.7
作者：
Kraeussling M;Wagner TU;Schartl M
通讯作者：
Schartl M