Site-Specific Augmentation of Amnion Cyclooxygenase-2 and Decidua Vera Phospholipase-A2 Expression in Labor: Possible Contribution of Mechanical Stretch and Interleukin-1 to Amnion Prostaglandin Synthesis
Site-Specific Augmentation of Amnion Cyclooxygenase-2 and Decidua Vera Phospholipase-A2 Expression in Labor: Possible Contribution of Mechanical Stretch and Interleukin-1 to Amnion Prostaglandin Synthesis
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临产时羊膜环加氧酶 2 和蜕膜磷脂酶 A2 表达的位点特异性增强：机械拉伸和白细胞介素 1 对羊膜前列腺素合成的可能贡献
DOI：
10.1177/107155760200900204
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
S. Fujii
中科院分区：
文献类型：
--
作者：
K. Terakawa;H. Itoh;N. Sagawa;S. Yura;Masahiro Yoshida;D. Korita;K. Kakui;S. Fujii
目的：探讨临产时胎膜前列腺素(PG)合成的可能部位特异性增强。方法：采用逆转录-聚合酶链式反应或免疫印迹法检测足月妊娠孕妇分娩前(n=8)和产后(n=24)羊膜、绒毛和蜕膜组织中胞浆磷脂酶A2(CPLA2)和环氧合酶-1、2(COX-1、-2)的表达。采用酶联免疫吸附试验检测羊膜源性Wish细胞经周期性机械拉伸和白细胞介素1(IL-1)刺激后前列腺素E_2(PGE2)的分泌。结果：所有标本均检测到cPLA2、COX-1和COX-2mRNAs表达。Western印迹分析显示，产后羊膜上部、绒毛膜和真皮蜕膜组织中COX-2的表达分别是产前的4.7倍、4.9倍和3.7倍(P均<0.05)。产后羊膜上部和绒毛组织中cPLA2蛋白的表达分别是产前的14.0倍和8.8倍(P均<0.05)。此外，在产后获取的标本中，羊膜COX-2和蜕膜真皮cPLA2在胎膜下部的表达分别是胎膜上部的1.9倍和2.6倍(P均<0.05)。在一项体内研究中，周期性机械拉伸显著增强了Wish细胞分泌IL-1增强的PGE2。结论：在羊膜和真性蜕膜组织的下部，靠近扩张的宫颈管内，PG的合成是产后特异的上调部位。羊膜PG合成的这种增强可能至少部分受IL-1和周期扩张的调节。
Objective: To investigate a possible site-specific augmentation of prostaglandin (PG) synthesis in the fetal membranes during labor. Methods: We used reverse transcriptase-polymerase chain reaction or Western blot analysis to evaluate the expression of cytosolic phopholipase A2 (cPLA2) and cyclooxygenase-1, -2 (COX-1, -2), in both the upper and lower parts of the amnion, chorion laeve, and decidua vera tissues from term pregnant women before (n = 8) and after labor (n = 24). Prostaglandin E2 (PGE2) secretion from amnion-derived WISH cells was assessed using enzyme-linked immunosorbent assay after stimulation by cyclic mechanical stretching and interleukin-1 (IL-1). Results: The expression of cPLA2 and COX-1 and COX-2 mRNAs was detected in all samples examined. Western blot analysis revealed that COX-2 expression in the upper part of the amnion, chorion laeve, and decidua vera tissues after labor was 4.7-, 4.9-, and 3.7-fold higher than that before labor, respectively (P < .05 for all). The cPLA2 protein expression in the upper part of the amnion and chorion laeve tissues after labor was 14.0- and 8.8-fold higher than that before labor, respectively (P < .05 for both). Moreover, in specimens obtained after labor, the amnion COX-2 expression and the decidua vera cPLA2 expression in the lower part of the fetal membrane was 1.9- and 2.6-fold higher than the respective levels in the upper part (P < .05 for both). In an in vitor study, cyclic mechanical stretching significantly enhanced IL-1-augmented PGE2 secretion from WISH cells. Conclusion: In the lower part of the amnion and decidua vera tissues, adjacent to the dilating cervical canal, PG synthesis was upregulated site specifically after labor. Such enhancement of amnion PG synthesis might be regulated at least partly by IL-1 and cyclic distension.
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Interleukin-1 beta 可诱导人羊膜来源的 WISH 细胞中胞质磷脂酶 A2 的合成和活性以及前列腺素 E2 的释放。
DOI：
10.1016/0090-6980(95)00069-m
发表时间：
1995
期刊：
Prostaglandins
影响因子：
--
作者：
Xue,S;Brockman,DE;Slater,DM;Myatt,L
通讯作者：
Myatt,L
DOI：
10.1016/s0002-9378(97)70459-9
发表时间：
1997-07-01
影响因子：
9.8
作者：
Skannal, DG;Brockman, DE;Myatt, L
通讯作者：
Myatt, L
DOI：
10.1016/0002-9378(81)90312-4
发表时间：
1981-01-01
影响因子：
9.8
作者：
OKAZAKI, T;CASEY, ML;JOHNSTON, JM
通讯作者：
JOHNSTON, JM
孕妇在分娩前和分娩过程中阴道/宫颈液中的白介素-1β、-1α、-6 和前列腺素。
DOI：
10.1210/jcem.77.3.8370702
发表时间：
1993
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Cox,SM;King,MR;Casey,ML;MacDonald,PC
通讯作者：
MacDonald,PC
羊水中前列腺素 (PG) 的积累是分娩的后遗症，并不表明 PGE2 或 PGF2 α 在人类分娩起始过程中发挥作用。
DOI：
10.1210/jcem.76.5.8496326
发表时间：
1993
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
MacDonald,PC;Casey,ML
通讯作者：
Casey,ML