A stabilized microbial ecosystem of self-limiting bacteria using synthetic quorum-regulated lysis.
A stabilized microbial ecosystem of self-limiting bacteria using synthetic quorum-regulated lysis.
复制标题
DOI：
10.1038/nmicrobiol.2017.83
复制
发表时间：
2017-06-12
影响因子：
28.3
通讯作者：
Hasty J
中科院分区：
文献类型：
--
作者：
Scott SR;Din MO;Bittihn P;Xiong L;Tsimring LS;Hasty J
微生物生态学家越来越多地转向小型的合成生态系统，作为探索原生微生物组复杂性的还原工具。与此同时，合成生物学家已经从单细胞基因回路转向使用细胞间信号来控制整个种群。这些领域的交叉正在产生废物回收，工业发酵，生物修复和人类健康的新方法。这些应用都面临着经典生态学中众所周知的共同挑战;如果没有阻止生长较快的物种消灭生长较慢的物种的机制，生态系统的稳定性就不会出现。在这里，我们结合联合收割机正交群体感应系统和人口控制电路与不同的自限性生长动力学，以工程师的两个'ortholysis'电路能够保持一个稳定的共培养的代谢竞争性菌株在微流控装置。虽然在没有合成种群控制的两菌株生态中没有观察到成功的共培养，但“正解”设计将共培养率从0%显著增加到约80%。基于代理的和确定性的建模表明，我们的系统可以进行调整，以产生不同的动态，包括相移，反相或同步振荡以及稳定的稳态人口密度。“正解”方法建立了一个范例，通过开发稳定的竞争性微生物群落，而不需要工程的相互依赖性，构建合成生态。
Microbial ecologists are increasingly turning to small, synthesized ecosystems as a reductionist tool to probe the complexity of native microbiomes. Concurrently, synthetic biologists have gone from single-cell gene circuits to controlling whole populations using intercellular signaling. The intersection of these fields is giving rise to new approaches in waste recycling, industrial fermentation, bioremediation, and human health. These applications share a common challenge well known in classical ecology; stability of an ecosystem cannot arise without mechanisms that prohibit the faster growing species from eliminating the slower. Here, we combine orthogonal quorum sensing systems and a population control circuit with diverse self-limiting growth dynamics in order to engineer two ‘ortholysis’ circuits capable of maintaining a stable co-culture of metabolically competitive strains in microfluidic devices. While no successful co-cultures are observed in a two-strain ecology without synthetic population control, the ‘ortholysis’ design dramatically increases the co-culture rate from 0% to approximately 80%. Agent-based and deterministic modeling reveal that our system can be adjusted to yield different dynamics, including phase-shifted, anti-phase or synchronized oscillations as well as stable steady-state population densities. The ‘ortholysis’ approach establishes a paradigm for constructing synthetic ecologies by developing stable communities of competitive microbes without the need for engineered codependency.
登录
查看更多内容
影响因子：
8.6
作者：
Mather W;Mondragón-Palomino O;Danino T;Hasty J;Tsimring LS
通讯作者：
Tsimring LS
DOI：
10.1016/s0273-1177(99)00490-1
发表时间：
1999-01-01
期刊：
LIFE SCIENCES: ARTIFICIAL ECOSYSTEMS
影响因子：
--
作者：
Fulget, N;Poughon, L;Lasseur, C
通讯作者：
Lasseur, C
影响因子：
46.9
作者：
Pédelacq, JD;Cabantous, S;Waldo, GS
通讯作者：
Waldo, GS
影响因子：
16.6
作者：
De Roy, Karen;Marzorati, Massimo;Boon, Nico
通讯作者：
Boon, Nico
影响因子：
64.8
作者：
Gravel, Dominique;Bell, Thomas;Mouquet, Nicolas
通讯作者：
Mouquet, Nicolas