Genetically Encoded Self-Assembling Iron Oxide Nanoparticles as a Possible Platform for Cancer-Cell Tracking.
Genetically Encoded Self-Assembling Iron Oxide Nanoparticles as a Possible Platform for Cancer-Cell Tracking.
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DOI：
10.3390/pharmaceutics13030397
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发表时间：
2021-03-16
期刊：
影响因子：
5.4
通讯作者：
Abakumov MA
中科院分区：
文献类型：
--
作者：
Efremova MV;Bodea SV;Sigmund F;Semkina A;Westmeyer GG;Abakumov MA
The study of growth and possible metastasis in animal models of tumors would benefit from reliable cell labels for noninvasive whole-organism imaging techniques such as magnetic resonance imaging. Genetically encoded cell-tracking reporters have the advantage that they are contrast-selective for viable cells with intact protein expression machinery. Besides, these reporters do not suffer from dilution during cell division. Encapsulins, which are bacterial protein nanocompartments, can serve as genetically controlled labels for multimodal detection of cells. Such nanocompartments can host various guest molecules inside their lumen. These include, for example, fluorescent proteins or enzymes with ferroxidase activity leading to biomineralization of iron oxide inside the encapsulin nanoshell. The aim of this work was to implement heterologous expression of encapsulin systems from Quasibacillus thermotolerans using the fluorescent reporter protein mScarlet-I and ferroxidase IMEF in the human hepatocellular carcinoma cell line HepG2. The successful expression of self-assembled encapsulin nanocompartments with functional cargo proteins was confirmed by fluorescence microscopy and transmission electron microscopy. Also, coexpression of encapsulin nanoshells, ferroxidase cargo, and iron transporter led to an increase in T2-weighted contrast in magnetic resonance imaging of HepG2 cells. The results demonstrate that the encapsulin cargo system from Q. thermotolerans may be suitable for multimodal imaging of cancer cells and could contribute to further in vitro and in vivo studies.
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影响因子：
16.6
作者：
Devaraj, Neal K.;Upadhyay, Rabi;Hatin, Jered B.;Hilderbrand, Scott A.;Weissleder, Ralph
通讯作者：
Weissleder, Ralph
影响因子：
9.7
作者：
Cheung, JYN;Ong, RCY;Kong, SK
通讯作者：
Kong, SK
DOI：
10.2967/jnumed.108.053546
发表时间：
2009-02
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
Budde MD;Frank JA
通讯作者：
Frank JA
影响因子：
--
作者：
Chen Q;Shang W;Zeng C;Wang K;Liang X;Chi C;Liang X;Yang J;Fang C;Tian J
通讯作者：
Tian J
影响因子：
4.8
作者：
Maynard-Smith, Lystranne A.;Chen, Ling-Chun;Wandless, Thomas J.
通讯作者：
Wandless, Thomas J.