Vitamin B and Vitamin C Affect DNA Methylation and Amino Acid Metabolism in Mycobacterium bovis BCG
Vitamin B and Vitamin C Affect DNA Methylation and Amino Acid Metabolism in Mycobacterium bovis BCG
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维生素 B 和维生素 C 影响牛分枝杆菌 BCG 的 DNA 甲基化和氨基酸代谢
DOI：
10.3389/fmicb.2020.00812
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发表时间：
2020-04
影响因子：
5.2
通讯作者：
Siguo Liu
中科院分区：
文献类型：
--
作者：
Ningning Song;Yongqiang Zhu;Yingying Cui;Mingyue LV;Yiyi Tang;Ziyin Cui;Guanghui Dang;Huajun Zheng;Siguo Liu
维生素是人体必需的营养物质，也是调节细胞代谢和激活免疫系统的酶的关键辅助因子。最近的研究表明，维生素B1 （VB1）和维生素C （Vc）可以抑制结核分枝杆菌的生长，但其确切机制尚不清楚。在本研究中，我们利用rna测序（RNA-seq）、液相色谱-质谱联用（LC-MS）和单分子实时测序（SMRT）分析了牛分枝杆菌BCG在VB1和Vc作用下的转录、代谢和甲基化谱。我们的研究结果表明，在维生素处理后，变异代谢物主要聚集在与氨基酸代谢相关的途径中。两种维生素处理均显著上调编码半胱氨酸合成酶a的基因。此外，只有用VC处理的卡介苗出现m4c修饰。携带该甲基化的基因被上调，表明m4c甲基化在一定程度上促进基因转录。总的来说，这项研究有助于了解VB1和VC的作用，并表明这些维生素是潜在的抗结核药物。
Vitamins are essential nutrients and key cofactors of enzymes that regulate cellular metabolism, and also activate the immune system. Recent studies have shown that vitamin B1 (VB1) and vitamin C (Vc) can inhibit Mycobacterium tuberculosis growth, but the precise mechanism is still not well understood. In the present study, we have used RNA-sequencing (RNA-seq), liquid chromatography coupled to mass spectrometry (LC-MS) and single-molecule real-time (SMRT) sequencing to analyze the transcriptional, metabolic and methylation profiles of Mycobacterium bovis BCG when treated with VB1 and Vc. Our results show that, after vitamin treatment, variant metabolites were mainly clustered in pathways related to amino acid metabolism. Treatment with both vitamins significantly up-regulated the gene encoding cysteine synthase A. Additionally, only BCG that was treated with VC showed m4c modifications. Genes harboring this methylation were up-regulated, suggesting that m4c methylation can promote gene transcription to some extent. Overall, this study contributes to the understanding of the effects of VB1 and VC, and suggests that these vitamins constitute potential anti-tuberculosis drugs.
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影响因子：
3
作者：
Wendl MC
通讯作者：
Wendl MC
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
巨噬细胞中结核分枝杆菌的转录适应：对吞噬体环境的见解。
影响因子：
15.3
作者：
Schnappinger, D;Ehrt, S;Voskuil, MI;Liu, Y;Mangan, JA;Monahan, IM;Dolganov, G;Efron, B;Butcher, PD;Nathan, C;Schoolnik, GK
通讯作者：
Schoolnik, GK
影响因子：
48
作者：
Flusberg, Benjamin A.;Webster, Dale R.;Lee, Jessica H.;Travers, Kevin J.;Olivares, Eric C.;Clark, Tyson A.;Korlach, Jonas;Turner, Stephen W.
通讯作者：
Turner, Stephen W.
影响因子：
4.4
作者：
Batra SD;Nandi M;Sikri K;Tyagi JS
通讯作者：
Tyagi JS