Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism.
Global Metabolic Profiling of Baculovirus Infection in Silkworm Hemolymph Shows the Importance of Amino-Acid Metabolism.
复制标题
蚕血淋巴中杆状病毒感染的整体代谢谱显示了氨基酸代谢的重要性。
DOI：
10.3390/v13050841
复制
发表时间：
2021-05-06
期刊：
影响因子：
--
通讯作者：
Sun J
中科院分区：
文献类型：
--
作者：
Feng M;Fei S;Xia J;Zhang M;Wu H;Swevers L;Sun J
病毒依靠宿主细胞代谢为成功的病毒复制提供必要的能量和生物合成前体。 Bombyx Mori Nucleopolyhedrovirus（BMNPV）对蚕的感染，Bombyx Mori，过去已广泛研究了杆菌病毒与其鳞翅目宿主之间的相互作用。为了了解宿主代谢反应与BMNPV感染之间的相互作用，我们分析了与BMNPV感染相关的全球代谢变化。我们的代谢分析数据表明，在BMNPV感染的时间过程中，氨基酸代谢发生了明显改变。在感染后24小时内BMNPV感染期间，氨基酸的消耗量增加（HPI），但它们的丰度在72 hpi时恢复。另一方面，中央碳代谢，尤其是糖酵解和谷氨酰胺分解，在BMNPV感染过程中未显示明显的变化。在药理上抑制糖酵解途径和谷氨酰胺溶解也未能减少BMNPV复制，这表明在BMNPV感染期间，糖酵解和谷氨酰胺溶解并不是必需的。这项研究揭示了在BMNPV感染期间实施的独特氨基酸利用过程。我们对BMNPV感染的蚕的代谢组学分析提供了有关杆状病毒在全身感染期间宿主代谢的改变的见解。
Viruses rely on host cell metabolism to provide the necessary energy and biosynthetic precursors for successful viral replication. Infection of the silkworm, Bombyx mori, by Bombyx mori nucleopolyhedrovirus (BmNPV), has been studied extensively in the past to unravel interactions between baculoviruses and their lepidopteran hosts. To understand the interaction between the host metabolic responses and BmNPV infection, we analyzed global metabolic changes associated with BmNPV infection in silkworm hemolymph. Our metabolic profiling data suggests that amino acid metabolism is strikingly altered during a time course of BmNPV infection. Amino acid consumption is increased during BmNPV infection at 24 h post infection (hpi), but their abundance recovered at 72 hpi. Central carbon metabolism, on the other hand, particularly glycolysis and glutaminolysis, did not show obvious changes during BmNPV infection. Pharmacologically inhibiting the glycolytic pathway and glutaminolysis also failed to reduce BmNPV replication, revealing that glycolysis and glutaminolysis are not essential during BmNPV infection. This study reveals a unique amino acid utilization process that is implemented during BmNPV infection. Our metabolomic analysis of BmNPV-infected silkworm provides insights as to how baculoviruses induce alterations in host metabolism during systemic infection.
登录
查看更多内容
影响因子：
7.3
作者：
Lin, Yu-Hsien;Tai, Chia-Chi;Wu, Yueh-Lung
通讯作者：
Wu, Yueh-Lung
影响因子：
1.2
作者：
Dionne, Marc S.
通讯作者：
Dionne, Marc S.
影响因子：
5.4
作者：
Sanchez, Erica L.;Pulliam, Thomas H.;Lagunoff, Michael
通讯作者：
Lagunoff, Michael
影响因子：
5.4
作者：
Fontaine, Krystal A.;Sanchez, Erica L.;Lagunoff, Michael
通讯作者：
Lagunoff, Michael
影响因子：
6.8
作者：
Kim, JJP;Battaile, KP
通讯作者：
Battaile, KP