Electric knife for cell surgery: Local ablation by micro-plasma discharge
Electric knife for cell surgery: Local ablation by micro-plasma discharge
复制标题
细胞手术用电刀：微等离子体放电局部消融
DOI：
--
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
F. Arai
中科院分区：
文献类型：
--
作者：
Y. Yamanishi;H. Kuriki;S. Sakuma;K. Onda;T. Kawahara;F. Arai
我们首次成功地应用微电刀对卵母细胞进行了去核。控制放电输出功率和导电面积，以适应水下和大气压环境下的细胞级烧蚀。利用银膏对铜线进行玻壳绝缘，实现了铜线的局部烧蚀，防止了铜线在水下放电时产生气泡和蛋白质污染。与传统电极相比，通过使用玻璃外壳绝缘体，消融区域的宽度成功地减小到一半以上。Hoechst染色的细胞核也被成功地去除，损伤区域有限，这是玻璃毛细管手工操作的困难任务。这种低成本的微电刀有可能扩展到在各种环境下制造任何目标材料，并为微纳米生物工程领域提供了一种新的自顶向下的制造方法。
We have firstly succeeded in enucleation of oocyte by using micro-electric knife. The discharged output power and conductive area were controlled to adapt the cellular-order ablation under water and under atmospheric pressure environment. The local area ablation was obtained by the glass shell insulation around the copper wire with silver paste, and which prevent the bubble generation and protein contamination when it was discharged under the water. The width of the ablation region was successfully reduced to more than a half by using the glass shell insulation compare to the conventional electrode. Moreover, the nucleus dyed by Hoechst was also removed successfully with limited damage region which is the difficult task for manual operation with glass capillary. This low cost micro-electric knife has a possibility to extend to fabricate any objective material under various environments and contribute to a new top-down fabrication method in the micro-nano bioengineering field.
用于眼内手术的脉冲电子雪崩刀（PEAK）。
DOI：
--
发表时间：
2001
期刊：
Investigative ophthalmology & visual science.
影响因子：
--
作者：
Palanker,DV;Miller,JM;Marmor,MF;Sanislo,SR;Huie,P;Blumenkranz,MS
通讯作者：
Blumenkranz,MS
影响因子：
6.1
作者：
Zeng, Fei;Rohde, Christopher B.;Yanik, Mehmet Fatih
通讯作者：
Yanik, Mehmet Fatih