Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes).
Genotyping-By-Sequencing (GBS) Detects Genetic Structure and Confirms Behavioral QTL in Tame and Aggressive Foxes (Vulpes vulpes).
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DOI：
10.1371/journal.pone.0127013
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kukekova AV
中科院分区：
文献类型：
--
作者：
Johnson JL;Wittgenstein H;Mitchell SE;Hyma KE;Temnykh SV;Kharlamova AV;Gulevich RG;Vladimirova AV;Fong HW;Acland GM;Trut LN;Kukekova AV
银狐（Vulpesvulpes）为研究社会行为和动物驯化的遗传学提供了一个新的模型。对狐狸的选择，分别为驯服和攻击行为，产生了两个品系，具有明显不同的，遗传决定的，行为表型。驯服品系的狐狸渴望与人建立联系，而攻击性品系的狐狸则具有攻击性，难以控制。这些菌株作为单独的远交系已经保持了40多代，但它们的遗传结构以前没有被研究过。我们应用基因分型测序（GBS）的方法，提供这些狐狸种群的遗传组成的见解。对驯服和攻击性狐狸的EcoT22I基因组文库的序列分析鉴定了48，294个高质量的SNP。种群结构分析表明，两个品系之间存在遗传分化，攻击品系的遗传多样性高于驯服品系。68个SNPs的等位基因频率在菌株之间存在显著差异。这些SNPs中的三个位于狐狸14号染色体上，在先前确定的行为QTL的间隔内，进一步支持该区域对行为的重要性。GBS SNP数据证实，尽管经过多年的选择性育种，但两个狐狸种群都保留了显着的遗传多样性。SNP等位基因频率在两个群体中的分析确定了几个区域的驯服和侵略性的狐狸之间的遗传分歧，其中一些可能代表的选择行为的目标。在这项研究中使用的GBS协议显着扩大了狐狸的基因组资源，并可以适用于SNP的发现和其他犬科物种的基因分型。
The silver fox (Vulpes vulpes) offers a novel model for studying the genetics of social behavior and animal domestication. Selection of foxes, separately, for tame and for aggressive behavior has yielded two strains with markedly different, genetically determined, behavioral phenotypes. Tame strain foxes are eager to establish human contact while foxes from the aggressive strain are aggressive and difficult to handle. These strains have been maintained as separate outbred lines for over 40 generations but their genetic structure has not been previously investigated. We applied a genotyping-by-sequencing (GBS) approach to provide insights into the genetic composition of these fox populations. Sequence analysis of EcoT22I genomic libraries of tame and aggressive foxes identified 48,294 high quality SNPs. Population structure analysis revealed genetic divergence between the two strains and more diversity in the aggressive strain than in the tame one. Significant differences in allele frequency between the strains were identified for 68 SNPs. Three of these SNPs were located on fox chromosome 14 within an interval of a previously identified behavioral QTL, further supporting the importance of this region for behavior. The GBS SNP data confirmed that significant genetic diversity has been preserved in both fox populations despite many years of selective breeding. Analysis of SNP allele frequencies in the two populations identified several regions of genetic divergence between the tame and aggressive foxes, some of which may represent targets of selection for behavior. The GBS protocol used in this study significantly expanded genomic resources for the fox, and can be adapted for SNP discovery and genotyping in other canid species.
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影响因子：
3.7
作者：
Baird NA;Etter PD;Atwood TS;Currey MC;Shiver AL;Lewis ZA;Selker EU;Cresko WA;Johnson EA
通讯作者：
Johnson EA
影响因子：
3.1
作者：
BELYAEV, DK
通讯作者：
BELYAEV, DK
影响因子：
4.5
作者：
Johansson AM;Pettersson ME;Siegel PB;Carlborg O
通讯作者：
Carlborg O
影响因子：
3.7
作者：
Elshire RJ;Glaubitz JC;Sun Q;Poland JA;Kawamoto K;Buckler ES;Mitchell SE
通讯作者：
Mitchell SE
影响因子：
4.5
作者：
Freedman AH;Gronau I;Schweizer RM;Ortega-Del Vecchyo D;Han E;Silva PM;Galaverni M;Fan Z;Marx P;Lorente-Galdos B;Beale H;Ramirez O;Hormozdiari F;Alkan C;Vilà C;Squire K;Geffen E;Kusak J;Boyko AR;Parker HG;Lee C;Tadigotla V;Wilton A;Siepel A;Bustamante CD;Harkins TT;Nelson SF;Ostrander EA;Marques-Bonet T;Wayne RK;Novembre J
通讯作者：
Novembre J