Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs.
Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs.
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DOI：
10.3390/antiox10101594
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发表时间：
2021-10-12
期刊：
影响因子：
--
通讯作者：
Jin JX
中科院分区：
文献类型：
--
作者：
Yin Z;Sun JT;Cui HD;Jiang CQ;Zhang YT;Lee S;Liu ZH;Jin JX
为研究单宁（TA）对猪卵母细胞体外成熟（IVM）的影响，在成熟培养基中添加不同浓度的TA(0、1、10和100 μg/mL)，观察其COCs和随后的胚胎发育情况。结果表明，与对照组相比，10µg/mL TA显著提高了卵丘扩张指数（CEI）、卵丘扩张相关基因（PTGS1、PTGS2、PTX-3、TNFAIP6和HAS2）的表达和孤雌激活（PA）、体外受精（IVF）和体细胞核移植（SCNT）后的囊胚形成率，但对卵母细胞核成熟没有影响。然而，10µg/mL TA显著提高了猪卵母细胞发育相关基因（BMP15、GDF9、CDC2和CYCLIN B1）、GSH、ATP、SOD1、PGC1α、BMP15、GDF9和CDC2的mRNA表达水平，并降低了细胞内ROS水平。结果表明，10µg/mL TA能促进猪卵母细胞的成熟。而高浓度TA （100 μg/mL）显著降低卵丘细胞CEI和PTGS1、PTGS2、PTX-3、HAS2 mRNA的表达，降低卵母细胞核成熟度和囊胚总细胞数/囊胚数。总的来说，这些数据表明，添加10 μg/mL TA对猪卵母细胞细胞质成熟和随后的胚胎发育有有益的影响。
To investigate the effects of tannins (TA) on porcine oocyte in vitro maturation (IVM), different concentrations of TA (0, 1, 10 and 100 μg/mL) were supplemented with a maturation medium and the COCs and subsequent embryonic development were examined. The results showed that 10 µg/mL TA significantly improved the cumulus expansion index (CEI), cumulus-expansion-related genes (PTGS1, PTGS2, PTX-3, TNFAIP6 and HAS2) expression and blastocyst formation rates after parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) compared to the control groups, but not oocyte nuclear maturation. Nevertheless, 10 µg/mL TA dramatically enhanced the mRNA expression of oocyte-development-related genes (BMP15, GDF9, CDC2 and CYCLIN B1), GSH, ATP, SOD1, PGC1α, BMP15, GDF9 and CDC2 levels and reduced intracellular ROS level in porcine oocytes. These results indicated that porcine oocyte cytoplasmic maturation was improved by 10 µg/mL TA treatment during IVM. In contrast, a high concentration of TA (100 μg/mL) significantly decreased the CEI and PTGS1, PTGS2, PTX-3 and HAS2 mRNA expressions in cumulus cells, and reduced oocyte nuclear maturation and the total cell numbers/blastocyst. In general, these data showed that 10 μg/mL TA supplementation has beneficial effects on oocyte cytoplasmic maturation and subsequent embryonic development in pigs.
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影响因子：
1.7
作者：
Lin, Z-L;Li, Y-H;Kim, N-H
通讯作者：
Kim, N-H
影响因子：
2.5
作者：
CHEN, L;RUSSELL, PT;LARSEN, WJ
通讯作者：
LARSEN, WJ
影响因子：
3.3
作者：
Jin, J. X.;Lee, S.;Lee, B. C.
通讯作者：
Lee, B. C.
影响因子：
10.3
作者：
Lee, Sanghoon;Jin, Jun-Xue;Lee, Byeong Chun
通讯作者：
Lee, Byeong Chun
DOI：
10.1152/ajpendo.00469.2012
发表时间：
2013-03-01
影响因子：
5.1
作者：
Auclair, Sylvain;Uzbekov, Rustem;Uzbekova, Svetlana
通讯作者：
Uzbekova, Svetlana