Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila.
Roles for Rac1 and Cdc42 in planar polarization and hair outgrowth in the wing of Drosophila.
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DOI：
10.1083/jcb.135.5.1277
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发表时间：
1996-12
影响因子：
7.8
通讯作者：
Simons, K
中科院分区：
文献类型：
--
作者：
Eaton, S;Wepf, R;Simons, K
果蝇的翅膀上覆盖着一排远端指向的毛发。毛发始于每个翅上皮细胞的单个膜突起，其远端取向由突起在细胞周长上的单个远端部位的限制决定(Wong，L.和P.Adler)。1993年。J.细胞生物学。123：209-211。)。我们研究了CDC42和rac1在翼毛形成中的作用。我们发现，在延伸的毛发中，肌动蛋白的局部聚合需要CDC42。通过表达一个显性的负蛋白干扰CDC42的活性，既取消了局部的肌动蛋白聚合，也取消了毛发生长。相反，rac1对于限制毛发生长的部位很重要。表达显性负值rac1N17的细胞不能将突起限制在一个单一的位置，并产生多个翼毛。这种极性缺陷与连接肌动蛋白的组织紊乱有关，也与与连接区域密切相关的复杂微管网络的破坏有关。我们还发现，顶端连接和微管参与了毛发生长的结构方面。在毛发形成过程中，指向远端的顶端微管伸长并填满羽毛。随着毛发的拉长，连接蛋白在每个细胞的近端和远端边缘重组。
The wing of Drosophila melanogaster is covered by an array of distally pointing hairs. A hair begins as a single membrane outgrowth from each wing epithelial cell, and its distal orientation is determined by the restriction of outgrowth to a single distal site on the cell circumference (Wong, L., and P. Adler. 1993. J. Cell Biol. 123:209- 211.). We have examined the roles of Cdc42 and Rac1 in the formation of wing hairs. We find that Cdc42 is required for localized actin polymerization in the extending hair. Interfering with Cdc42 activity by expression of a dominant negative protein abolishes both localized actin polymerization and hair outgrowth. In contrast, Rac1 is important for restricting the site at which hairs grow out. Cells expressing the dominant negative Rac1N17 fail to restrict outgrowth to a single site and give rise to multiple wing hairs. This polarity defect is associated with disturbances in the organization of junctional actin and also with disruption of an intricate microtubule network that is intimately associated with the junctional region. We also find that apical junctions and microtubules are involved in structural aspects of hair outgrowth. During hair formation, the apical microtubules that point distally elongate and fill the emerging wing hair. As the hair elongates, junctional proteins are reorganized on the proximal and distal edges of each cell.
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影响因子：
2.7
作者：
LEMULLOIS, M;BOISVIEUXULRICH, E;SANDOZ, D
通讯作者：
SANDOZ, D
影响因子：
2.7
作者：
MITCHELL, HK;ROACH, J;PETERSEN, NS
通讯作者：
PETERSEN, NS
影响因子：
10.5
作者：
LUO, LQ;LIAO, YJ;JAN, YN
通讯作者：
JAN, YN
DOI：
10.1083/jcb.109.6.2817
发表时间：
1989-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bacallao R;Antony C;Dotti C;Karsenti E;Stelzer EH;Simons K
通讯作者：
Simons K
影响因子：
7.8
作者：
Cant, K;Knowles, B A;Mooseker, M S;Cooley, L
通讯作者：
Cooley, L