Applicability of next generation sequencing technology in microsatellite instability testing.
Applicability of next generation sequencing technology in microsatellite instability testing.
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DOI：
10.3390/genes6010046
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发表时间：
2015-02-12
期刊：
影响因子：
3.5
通讯作者：
Taylor G
中科院分区：
文献类型：
--
作者：
Gan C;Love C;Beshay V;Macrae F;Fox S;Waring P;Taylor G
Microsatellite instability (MSI) is a useful marker for risk assessment, prediction of chemotherapy responsiveness and prognosis in patients with colorectal cancer. Here, we describe a next generation sequencing approach for MSI testing using the MiSeq platform. Different from other MSI capturing strategies that are based on targeted gene capture, we utilize “deep resequencing”, where we focus the sequencing on only the microsatellite regions of interest. We sequenced a series of 44 colorectal tumours with normal controls for five MSI loci (BAT25, BAT26, BAT34c4, D18S55, D5S346) and a second series of six colorectal tumours (no control) with two mononucleotide loci (BAT25, BAT26). In the first series, we were able to determine 17 MSI-High, 1 MSI-Low and 26 microsatellite stable (MSS) tumours. In the second series, there were three MSI-High and three MSS tumours. Although there was some variation within individual markers, this NGS method produced the same overall MSI status for each tumour, as obtained with the traditional multiplex PCR-based method.
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